The pretreatment of lignocellulosic material performed to improve substrate’s susceptibility to enzymatic hydrolysis is usually accompanied by reactions leading to the synthesis of compounds that inhibit the metabolic activity of microorganisms. Their toxicity is the main obstacle to the successful bioconversion of lignocellulosic hydrolysates. The identification of these inhibitors and the choice of the optimal detoxication method are crucial for the improving the efficiency of fermentation processes. Material rinsing with water after processing is a common detoxication practice. However, it generates material losses, thus affecting contents of saccharides in the fermentation medium, which may in turn trigger higher costs of lignocellulose conversion to ethanol and other products with a higher added value. A study was undertaken to determine the effect of selected methods for the detoxication of an enzymatic hydrolysate from Miscanthus giganteus on the fermentation efficiency of saccharide derivatives. The experiment conducted with Mucor rouxii DSM 1191 demonstrated the usability of the detoxication method based on the activated carbon. After 96-h fermentation of Miscanthus hydrolysates, the alcohol content in the post-reaction medium was higher by 14% than in the control experiment wherein the material was rinsed with water after pretreatment. The experiment carried out with Saccharomyces cerevisiae 7, NRRL 978 showed no positive impact of the alternative detoxication methods replacing material rinsing on the efficiency of ethanol synthesis. The highest concentration of this metabolite (2.04% (v/v)) was obtained in the experimental variant in which the mentioned operation was coupled with detoxication of hydrolysates using calcium hydroxide.

为改善底物对酶水解的敏感性而进行的木质纤维素材料的预处理通常伴随有反应，这些反应导致合成抑制微生物代谢活性的化合物。它们的毒性是木质纤维素水解产物成功生物转化的主要障碍。这些抑制剂的鉴定和最佳脱毒方法的选择对于提高发酵过程的效率至关重要。加工后用水冲洗材料是一种常见的脱毒方法。但是，它会产生物质损失，从而影响发酵培养基中糖类的含量，进而可能引发将木质纤维素转化为乙醇和其他具有较高附加值的产品的较高成本。芒草对糖类衍生物的发酵效率。用Mucor rouxii DSM 1191进行的实验证明了基于活性炭的脱毒方法的可用性。芒草水解物发酵96小时后，反应后培养基中的酒精含量比对照实验高14％，在对照实验中，预处理后将材料用水冲洗。用酿酒酵母7进行的实验显示，NRRL 978替代脱毒方法代替材料冲洗对乙醇合成效率没有积极影响。该代谢物的最高浓度（2.04％（v/ v））是在实验变体中获得的，其中所述操作与使用氢氧化钙使水解产物脱毒相结合。