Abstract

Blowfly (Diptera: Calliphoridae) species Lucilia sericata (Meigen) and related species Lucilia cuprina (Wiedmann) are important agricultural pests, assist in forensic fields and also have a therapeutic role in medicine. Both species (though predominantly L. sericata) are utilised in a clinical setting for maggot debridement therapy (MDT) where the larvae ingest necrotic tissue and bacteria from non-healing wounds. Conversely, larvae of L. cuprina feed invasively, as major initiators of sheep myiasis in Australia, New Zealand, and the UK, among other regions. Both species exhibit larval and adult interactions with bacterially rich environments, but the significance of this in the composition of their microbiome has yet to be considered. This study utilised dissected samples of digestive and reproductive organs from both disinfected and non-disinfected adults and larvae of both species for bacterial DNA extraction, followed by 16S rRNA gene sequencing. Sequencing data indicated unsurprisingly that digestive tracts of both genders and female salivary glands from all non-disinfected samples carry the most concentrated amounts of bacteria. Genera Pseudomonas and Corynebacterium were also highly represented within all organs and species analysed. Comparison of bait lures to sample sequence read output of insect specimens showed no correlation with genera such as Pseudomonas present in insects, while absent from wild bait, and in reduced amounts from fleece bait profiles. With this information, future work can focus on key organs such as the spermathecae and salivary glands, while also providing the potential to identify the role these bacteria may play in the blowfly life cycle.

Key points

• Genera Pseudomonas appears consistently in the microbiome of Lucilia species.

• Female spermathecae and salivary glands show the highest microbial diversity.

• Bacterial profiles of L. sericata and L. cuprina have similar composition.

中文翻译：

---

通过 16S rRNA 基因测序观察丝光绿藻和铜绿藻器官的细菌关联

摘要

蝇蛆（双翅目：Calliphoridae）种丝光绿蝇（Meigen）和相关种绿光蝇（Wiedmann）是重要的农业害虫，在法医领域有帮助，在医学上也有治疗作用。这两个物种（虽然主要是丝光乳杆菌）都用于蛆清创治疗 (MDT) 的临床环境中，其中幼虫从不愈合的伤口中摄取坏死组织和细菌。相反，L. cuprina的幼虫侵入性饲料，在澳大利亚、新西兰和英国等地区是羊蝇蛆病的主要发起者。这两个物种都表现出与富含细菌的环境的幼虫和成虫相互作用，但这在其微生物组组成中的重要性尚待考虑。这项研究利用从两个物种的消毒和未消毒成虫和幼虫的消化和生殖器官的解剖样本进行细菌 DNA 提取，然后进行 16S rRNA 基因测序。测序数据不出所料地表明，来自所有未消毒样本的两性消化道和女性唾液腺都携带最集中的细菌量。假单胞菌属和棒状杆菌属在所分析的所有器官和物种中也有很高的代表性。诱饵与昆虫标本的样本序列读取输出的比较显示，与昆虫中存在的假单胞菌属等属没有相关性，而野生诱饵中不存在，并且羊毛诱饵轮廓中的数量减少。有了这些信息，未来的工作可以专注于关键器官，如精囊和唾液腺，同时也有可能确定这些细菌在苍蝇生命周期中可能发挥的作用。

关键点

• 假单胞菌属始终出现在 Lucilia 物种的微生物组中。

• 雌性受精囊和唾液腺显示出最高的微生物多样性。

• L. sericata 和 L. cuprina 的细菌特征具有相似的组成。