The diversity of antibiotic-resistance bacteria (ARB) from the effluents of Aizawl city municipal waste dumping site was studied using a culture-dependent method. The present study molecularly identified 73 isolates that were differentiated into three phyla (Proteobacteria, Actinobacteria, and Firmicutes) belonging to 17 family and 22 genera. Bacillus constitutes the most dominant genus comprising 16% of the total isolates. The topology of the phylogenetic tree differentiates them into five major clades. Corynebacterium and Rhodococcus which are morphologically alike were clustered together and the Gram-positive bacteria such as Staphylococcus, Bacillus, and Lysinibacillus formed a separate cluster. Antibiotic resistance of the identified bacterial isolates was performed using 19 different antibiotics. Among the isolates, 70 of them found resistant to polymixin B and nalidixic acid and 10 isolates exhibited resistance to 15 tested antibiotics. The present study revealed that bacteria with antibiotic resistance are extensively distributed in the effluents of the dumping site and may serve as a significant reservoir for the spreading of antibiotic resistance to opportunistic pathogens.

采用培养依赖性方法研究了艾藻尔市城市垃圾倾倒场污水中抗生素耐药菌 (ARB) 的多样性。本研究从分子上鉴定了 73 个分离物，这些分离物分为三个门（变形菌门、放线菌门和厚壁菌门），分别属于 17 个科和 22 个属。芽孢杆菌属占总分离株的 16%。系统发育树的拓扑结构将它们分为五个主要进化枝。形态相似的棒状杆菌和红球菌聚集在一起，而革兰氏阳性菌如葡萄球菌、芽孢杆菌和赖氨酸芽孢杆菌形成了一个单独的集群。使用 19 种不同的抗生素对已鉴定的细菌分离物进行抗生素耐药性。其中70株对多粘菌素B和萘啶酸耐药，10株对15种受试抗生素耐药。目前的研究表明，具有抗生素耐药性的细菌广泛分布在倾倒场的污水中，并且可能成为抗生素耐药性传播到机会性病原体的重要水库。