Background/Aim: Osteosarcoma is a rare type of malignancy that affects mostly children and adolescents. A new procedure was designed to create an improved patient-derived orthotopic xenograft (PDOX) mouse model of osteosarcoma that more closely mimics osteosarcoma in clinical settings. Previous osteosarcoma PDOX models involved implanting a tumor fragment near the femur of nude mice in a space created by separating muscle. Materials and Methods: A hole was created in the tibia of nude mice and an osteosarcoma tumor fragment was implanted directly into the bone. Results: This procedure resulted in tumor growth in the bone similar to osteosarcoma tumors found in clinical patients. Conclusion: The establishment ratio for this procedure is 80% making it a practical and clinically-relevant model for screening effective therapies for osteosarcoma patients.

中文翻译：

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一种用于建立更临床骨肉瘤 PDOX 小鼠模型的原位胫骨植入新程序

背景/目的：骨肉瘤是一种罕见的恶性肿瘤，主要影响儿童和青少年。设计了一种新程序来创建一种改进的源自患者的原位异种移植 (PDOX) 骨肉瘤小鼠模型，该模型更接近于临床环境中的骨肉瘤。以前的骨肉瘤 PDOX 模型涉及在裸鼠股骨附近通过分离肌肉产生的空间中植入肿瘤碎片。材料与方法：在裸鼠胫骨上开一个洞，将骨肉瘤肿瘤碎片直接植入骨骼中。结果：该程序导致骨中的肿瘤生长类似于临床患者中发现的骨肉瘤肿瘤。结论：