Background/Aim: Glucose transporter 1 (GLUT1) has been demonstrated to be overexpressed in various cancer tissues and play a significant role on growth, metastasis, and apoptosis in cancer cells. This study aimed to reveal the clinical relevance of glucose transporter 1 (GLUT1) in carcinogenesis and progression on liver fluke-associated cholangiocarcinoma (CCA). Materials and Methods: Expression of GLUT1 in CCA tissues from patients, as well as from a liver fluke-induced CCA hamster model, was determined using immunohistochemistry. CCA cell lines were transfected with GLUT1 siRNA and the roles of GLUT1 on cell growth as well as migration and invasion were investigated by using a clonogenic assay and Boyden chamber assays, respectively. Results: GLUT1 was aberrantly expressed in hyperplastic/dysplastic bile ducts and CCA, but not in the normal bile ducts. High GLUT1 expression was significantly associated with non-papillary type, large tumor size, and short survival of patients. GLUT1 was expressed during cholangio-carcinogenesis and gradually increased with progression of histopathologic bile ducts. Silencing of GLUT1 expression significantly suppressed growth, migration, and invasion of CCA cell lines. Conclusion: GLUT1 plays important roles in carcinogenesis and progression of liver fluke-associated CCA. Targeting GLUT1 may be a strategy for treatment of metastasis in liver fluke-associated CCA.

中文翻译：

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异常的 GLUT1 表达与肝吸虫相关胆管癌的癌变和进展有关

背景/目的：葡萄糖转运蛋白 1 (GLUT1) 已被证明在各种癌症组织中过度表达，并在癌细胞的生长、转移和凋亡中发挥重要作用。本研究旨在揭示葡萄糖转运蛋白 1 (GLUT1) 在肝吸虫相关胆管癌 (CCA) 的癌变和进展中的临床相关性。材料和方法： GLUT1 在患者的 CCA 组织以及肝吸虫诱导的 CCA 仓鼠模型中的表达，使用免疫组织化学进行测定。用 GLUT1 siRNA 转染 CCA 细胞系，并分别使用克隆形成试验和 Boyden 室试验研究 GLUT1 在细胞生长以及迁移和侵袭中的作用。结果：GLUT1在增生/发育不良胆管和CCA中异常表达，但不在正常的胆管中。高 GLUT1 表达与非乳头状型、大肿瘤大小和患者的短生存期显着相关。GLUT1 在胆管癌发生过程中表达，并随着组织病理学胆管的进展而逐渐增加。GLUT1 表达的沉默显着抑制了 CCA 细胞系的生长、迁移和侵袭。结论：GLUT1在肝吸虫相关CCA的发生和进展中起重要作用。靶向 GLUT1 可能是治疗肝吸虫相关 CCA 转移的一种策略。GLUT1 表达的沉默显着抑制了 CCA 细胞系的生长、迁移和侵袭。结论：GLUT1在肝吸虫相关CCA的发生和进展中起重要作用。靶向 GLUT1 可能是治疗肝吸虫相关 CCA 转移的一种策略。GLUT1 表达的沉默显着抑制了 CCA 细胞系的生长、迁移和侵袭。结论：GLUT1在肝吸虫相关CCA的发生和进展中起重要作用。靶向 GLUT1 可能是治疗肝吸虫相关 CCA 转移的一种策略。