Background: Enamel matrix derivative (EMD) is widely used for regeneration therapy in dental clinical situations, but the mechanism of EMD bioactivity remains obscure. To clarify this mechanism, we focused on the formation of connective tissue and blood vessels. The aim of this study was to confirm whether EMD induces the formation of connective tissue and blood vessels by using the diffusion chamber (DC) technique. Materials and Methods: Individual DCs containing EMD (DC-EMD) or propylene glycol alginate (PGA) were implanted subcutaneously in rat dorsum. At 4 weeks after the implantation, histological analysis of DCs was performed using azan staining. Results: DC-EMD induced the formation of much larger amounts of connective tissue containing abundant blood vessels than did DC-PGA. Conclusion: The results indicated that EMD can induce the formation of both connective tissue and blood vessels. This bioactivity may contribute to the mechanism whereby EMD induces tissue regeneration.

中文翻译：

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大鼠皮下植入扩散室中的釉质基质衍生物诱导含有丰富血管的纤维结缔组织的形成

背景：牙釉质基质衍生物（EMD）广泛用于牙科临床情况下的再生治疗，但 EMD 生物活性的机制仍不清楚。为了阐明这一机制，我们专注于结缔组织和血管的形成。本研究的目的是通过使用扩散室 (DC) 技术确认 EMD 是否诱导结缔组织和血管的形成。材料和方法：将含有 EMD (DC-EMD) 或海藻酸丙二醇酯 (PGA) 的单个 DC 植入大鼠背部皮下。在植入后 4 周，使用 azan 染色对 DCs 进行组织学分析。结果：与 DC-PGA 相比，DC-EMD 诱导了大量含有丰富血管的结缔组织的形成。结论：结果表明EMD可以诱导结缔组织和血管的形成。这种生物活性可能有助于 EMD 诱导组织再生的机制。