The mammalian heart is derived from multiple cell lineages; however, our understanding of when and how the diverse cardiac cell types arise is limited. We mapped the origin of the embryonic mouse heart at single-cell resolution using a combination of transcriptomic, imaging, and genetic lineage labeling approaches. This provided a transcriptional and anatomic definition of cardiac progenitor types. Furthermore, it revealed a cardiac progenitor pool that is anatomically and transcriptionally distinct from currently known cardiac progenitors. Besides contributing to cardiomyocytes, these cells also represent the earliest progenitor of the epicardium, a source of trophic factors and cells during cardiac development and injury. This study provides detailed insights into the formation of early cardiac cell types, with particular relevance to the development of cell-based cardiac regenerative therapies.

中文翻译：

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心外膜和心肌的共同祖细胞池的表征

哺乳动物的心脏来自多个细胞谱系；然而，我们对不同心脏细胞类型何时以及如何出现的理解是有限的。我们结合使用转录组学、成像和遗传谱系标记方法，以单细胞分辨率绘制了胚胎小鼠心脏的起源。这提供了心脏祖细胞类型的转录和解剖学定义。此外，它揭示了一个心脏祖细胞池，在解剖学和转录上与目前已知的心脏祖细胞不同。除了有助于心肌细胞，这些细胞还代表了心外膜的最早祖细胞，是心脏发育和损伤过程中营养因子和细胞的来源。这项研究提供了对早期心肌细胞类型形成的详细见解，