To investigate the complexity of proteomics in cervical cancer tissues, we used isobaric tags for relative and absolute quantitation (iTRAQ)-based mass spectrometry analysis on a panel of normal cervical tissues (N), high-grade squamous intraepithelial lesion tissues (HSIL) and cervical cancer tissues (CC). Total 72 differentially expressed proteins were identified both in CC vs N and CC vs HSIL. The expression of HMGB2 was markedly higher in CC than that in HSIL and N. High HMGB2 expression was significantly correlated with primary tumor size, invasion and tumor stage. The up-regulated HMGB2 was discovered to be associated with human cervical cancer. These findings suggest that HMGB2 may be a potentially prognostic biomarker and a target for the therapy of cervical cancer.

中文翻译：

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HMGB2差异表达蛋白的鉴定及其在宫颈癌中的临床病理意义

为了研究蛋白质组学在子宫颈癌组织中的复杂性，我们使用等压标记对一组正常子宫颈组织（N），高级鳞状上皮内病变组织（HSIL）和相对定量进行基于绝对定量（iTRAQ）的质谱分析子宫颈癌组织（CC）。在CC vs N和CC vs HSIL中共鉴定出72种差异表达的蛋白质。CC中HMGB2的表达明显高于HSIL和N。HMGB2的高表达与原发肿瘤的大小，侵袭和肿瘤的分期显着相关。发现上调的HMGB2与人宫颈癌有关。这些发现表明，HMGB2可能是潜在的预后生物标志物，并且是宫颈癌治疗的靶标。