当前位置: X-MOL 学术BMC Plant Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Comparative plastid genomics of four Pilea (Urticaceae) species: insight into interspecific plastid genome diversity in Pilea
BMC Plant Biology ( IF 5.3 ) Pub Date : 2021-01-07 , DOI: 10.1186/s12870-020-02793-7
Jingling Li 1 , Jianmin Tang 2 , Siyuan Zeng 1 , Fang Han 1 , Jing Yuan 1 , Jie Yu 1, 3
Affiliation  

Pilea is a genus of perennial herbs from the family Urticaceae, and some species are used as courtyard ornamentals or for medicinal purposes. At present, there is no information about the plastid genome of Pilea, which limits our understanding of this genus. Here, we report 4 plastid genomes of Pilea taxa (Pilea mollis, Pilea glauca ‘Greizy’, Pilea peperomioides and Pilea serpyllacea ‘Globosa’) and performed comprehensive comparative analysis. The four plastid genomes all have a typical quartile structure. The lengths of the plastid genomes ranged from 150,398 bp to 152,327 bp, and each genome contained 113 unique genes, including 79 protein-coding genes, 4 rRNA genes, and 30 tRNA genes. Comparative analysis showed a rather high level of sequence divergence in the four genomes. Moreover, eight hypervariable regions were identified (petN-psbM, psbZ-trnG-GCC, trnT-UGU-trnL-UAA, accD-psbI, ndhF-rpl32, rpl32-trnL-UAG, ndhA-intron and ycf1), which are proposed for use as DNA barcode regions. Phylogenetic relationships based on the plastid genomes of 23 species of 14 genera of Urticaceae resulted in the placement of Pilea in the middle and lower part of the phylogenetic tree, with 100% bootstrap support within Urticaceae. Our results enrich the resources concerning plastid genomes. Comparative plastome analysis provides insight into the interspecific diversity of the plastid genome of Pilea. The identified hypervariable regions could be used for developing molecular markers applicable in various research areas.

中文翻译:

四种菌毛(荨麻科)物种的质体比较基因组学:对菌毛种间质体基因组多样性的洞察

菌毛是荨麻科多年生草本植物,有些种类用作庭院观赏植物或药用。目前还没有关于菌毛的质体基因组的信息,限制了我们对该属的了解。在这里,我们报告了菌毛类群(Pilea mollis、Pilea glauca 'Greizy'、Pilea peperomioides 和 Pilea serpyllacea 'Globosa')的 4 个质体基因组,并进行了全面的比较分析。四个质体基因组都具有典型的四分位结构。质体基因组的长度从150,398 bp到152,327 bp不等,每个基因组包含113个独特的基因,包括79个蛋白质编码基因、4个rRNA基因和30个tRNA基因。比较分析显示四个基因组的序列差异水平相当高。此外,还鉴定了八个高变区(petN-psbM、psbZ-trnG-GCC、trnT-UGU-trnL-UAA、accD-psbI、ndhF-rpl32、rpl32-trnL-UAG、ndhA-内含子和 ycf1),建议用作 DNA 条形码区域。基于荨麻科 14 属 23 种的质体基因组的系统发育关系导致菌毛位于系统发育树的中部和下部,在荨麻科内具有 100% 的引导支持。我们的结果丰富了有关质体基因组的资源。比较质体分析提供了对菌毛质体基因组种间多样性的深入了解。鉴定出的高变区可用于开发适用于各个研究领域的分子标记。基于荨麻科 14 属 23 种的质体基因组的系统发育关系导致菌毛位于系统发育树的中部和下部,在荨麻科内具有 100% 的引导支持。我们的结果丰富了有关质体基因组的资源。比较质体分析提供了对菌毛质体基因组种间多样性的深入了解。鉴定出的高变区可用于开发适用于各个研究领域的分子标记。基于荨麻科 14 属 23 种的质体基因组的系统发育关系导致菌毛位于系统发育树的中部和下部,在荨麻科内具有 100% 的引导支持。我们的结果丰富了有关质体基因组的资源。比较质体分析提供了对菌毛质体基因组种间多样性的深入了解。鉴定出的高变区可用于开发适用于各个研究领域的分子标记。比较质体分析提供了对菌毛质体基因组种间多样性的深入了解。鉴定出的高变区可用于开发适用于各个研究领域的分子标记。比较质体分析提供了对菌毛质体基因组种间多样性的深入了解。鉴定出的高变区可用于开发适用于各个研究领域的分子标记。
更新日期:2021-01-07
down
wechat
bug