Consolidated bioprocessing using oleaginous yeast is a promising modality for the economic conversion of plant biomass to fuels and chemicals. However, yeast are not known to produce effective biomass degrading enzymes naturally and this trait is essential for efficient consolidated bioprocessing. We expressed a chimeric cellobiohydrolase I gene in three different oleaginous, industrially relevant yeast: Yarrowia lipolytica, Lipomyces starkeyi, and Saccharomyces cerevisiae to study the biochemical and catalytic properties and biomass deconstruction potential of these recombinant enzymes. Our results showed differences in glycosylation, surface charge, thermal and proteolytic stability, and efficacy of biomass digestion. L. starkeyi was shown to be an inferior active cellulase producer compared to both the Y. lipolytica and S. cerevisiae enzymes, whereas the cellulase expressed in S. cerevisiae displayed the lowest activity against dilute-acid-pretreated corn stover. Comparatively, the chimeric cellobiohydrolase I enzyme expressed in Y. lipolytica was found to have a lower extent of glycosylation, better protease stability, and higher activity against dilute-acid-pretreated corn stover.

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嵌合纤维二糖水解酶I在三种含油酵母中的表达，活性和生化特性

使用油质酵母进行的整合生物处理是一种将植物生物质经济转化为燃料和化学品的有前途的方式。然而，未知酵母会自然产生有效的生物质降解酶，而这一特性对于有效的整合生物处理至关重要。我们在三种不同的含油的，与工业相关的酵母中表达了一种嵌合纤维二糖水解酶I基因：解脂耶氏酵母，脂单胞菌starkeyi和酿酒酵母，以研究这些重组酶的生化和催化特性以及生物质解构潜力。我们的结果显示了糖基化，表面电荷，热和蛋白水解稳定性以及生物质消化功效的差异。与解脂耶氏酵母和酿酒酵母相比，starkeyi乳酸菌的活性纤维素生产能力较差，而在酿酒酵母中表达的纤维素酶对用稀酸预处理的玉米秸秆表现出最低的活性。相比之下，发现在解脂耶氏酵母中表达的嵌合纤维二糖水解酶I酶具有较低的糖基化程度，更好的蛋白酶稳定性和更高的抗稀酸预处理玉米秸秆的活性。