Cutaneotrichosporon oleaginosus ATCC 20509 is a fast-growing oleaginous basidiomycete yeast that is able to grow in a wide range of low-cost carbon sources including crude glycerol, a byproduct of biodiesel production. When glycerol is used as a carbon source, this yeast can accumulate more than 50% lipids (w/w) with high concentrations of mono-unsaturated fatty acids. To increase our understanding of this yeast and to provide a knowledge base for further industrial use, a FAIR re-annotated genome was used to build a genome-scale, constraint-based metabolic model containing 1553 reactions involving 1373 metabolites in 11 compartments. A new description of the biomass synthesis reaction was introduced to account for massive lipid accumulation in conditions with high carbon-to-nitrogen (C/N) ratio in the media. This condition-specific biomass objective function is shown to better predict conditions with high lipid accumulation using glucose, fructose, sucrose, xylose, and glycerol as sole carbon source. Contributing to the economic viability of biodiesel as renewable fuel, C. oleaginosus ATCC 20509 can effectively convert crude glycerol waste streams in lipids as a potential bioenergy source. Performance simulations are essential to identify optimal production conditions and to develop and fine tune a cost-effective production process. Our model suggests ATP-citrate lyase as a possible target to further improve lipid production.

中文翻译：

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基因组规模代谢模型强调了 Cutaneotrichosporon oleaginosus ATCC 20509 作为生物燃料生产细胞工厂的潜力

Cutaneotrichosporon oleaginosus ATCC 20509 是一种快速生长的产油担子菌酵母，能够在多种低成本碳源中生长，包括粗甘油（生物柴油生产的副产品）。当使用甘油作为碳源时，这种酵母可以积累超过 50% 的脂质（w/w）以及高浓度的单不饱和脂肪酸。为了增加我们对这种酵母的了解并为进一步的工业用途提供知识库，使用 FAIR 重新注释的基因组来构建基因组规模、基于约束的代谢模型，其中包含 11 个区室中涉及 1373 种代谢物的 1553 个反应。引入了生物质合成反应的新描述，以解释介质中高碳氮 (C/N) 比条件下的大量脂质积累。这种特定条件的生物量目标函数被证明可以更好地预测使用葡萄糖、果糖、蔗糖、木糖和甘油作为唯一碳源的高脂质积累条件。C. oleaginosus ATCC 20509 有助于将生物柴油作为可再生燃料的经济可行性，有效地将粗甘油废物流转化为脂质，作为潜在的生物能源。性能模拟对于确定最佳生产条件以及开发和微调具有成本效益的生产流程至关重要。我们的模型表明 ATP-柠檬酸裂解酶作为进一步提高脂质生产的可能目标。