The preventive and therapeutic medical utilization of this plant is an age-long practice across the globe. This study aimed to validate the impact of dark purple blossoms of basil (Ocimum basilicum L.) aqueous extract at low temperature (0 °C) mediated mitochondrial fission contributed to induced apoptosis in human breast cancer cells. Fresh blossoms were extracted at low temperature (0 °C) using a watery solvent. Human MCF7 breast cancer cells were then treated with 3 separate fluctuated concentrations of 0, 50, 150 and 250 µg/mL for 24 and 48 h. The outcomes demonstrated the presence of anthocyanins, anthraquinones, tannins, reducing sugars, glycosides, proteins, amino acids, flavonoids and volatile oils and nonappearance of Terpinoids and alkaloids. Contrastingly, frail presence of steroids in basil blossoms aqueous concentrate was noted. In addition, the results from a phytochemical subjective examination of basil (Ocimum basilicum L.) blossoms aqueous extract demonstrated that most of the credited natural impacts containing more remarkable contents of antioxidants and anticancer compounds in basil blossoms aqueous extract. Moreover, the restraint of glucose take-up was alleviated mediated by a dose-dependent manner in MCF7 cells with basil (Ocimum basilicum L.) blossoms aqueous extract inducted for 24 h, resulting in mitochondrial fission. This is the first study that shows the impact of the aqueous extract of basil (Ocimum basilicum L.) blossoms was extracted at low temperature (0℃/6 h) underlined high amounts of flavonoids and phenolic compounds bearing more anticancer and antioxidant activities compared to another aqueous extract (using boiled water solvent) and alcoholic extracts.

中文翻译：

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罗勒（Ocimum basilicum L.）的紫色开花的低温提取物干预线粒体易位有助于促使人乳腺癌细胞凋亡。

这种植物的预防性和治疗性医学利用在全球范围内已有很长的历史。这项研究旨在验证罗勒（Ocimum basilicum L.）水提物在低温（0°C）介导的线粒体裂变中暗紫色开花对人乳腺癌细胞诱导凋亡的影响。使用水性溶剂在低温（0°C）下提取新鲜花朵。然后，将人MCF7乳腺癌细胞分别以3、0、50、150和250 µg / mL的波动浓度处理24和48小时。结果表明存在花青素，蒽醌，单宁，还原糖，糖苷，蛋白质，氨基酸，类黄酮和挥发油，并且不存在萜类和生物碱。相反，注意到罗勒花浓缩水溶液中类固醇的脆弱存在。此外，罗勒（Ocimum basilicum L.）花朵水提物的植物化学主观检查结果表明，大多数值得称道的自然影响都包含罗勒花朵水提物中的抗氧化剂和抗癌化合物含量更高。此外，MCF7细胞中的罗勒（Ocimum basilicum L.）开花水提取物诱导24 h，通过剂量依赖的方式减轻了对葡萄糖吸收的抑制，导致线粒体裂变。这是第一项显示罗勒（Ocimum basilicum L.