Bacteria belonging to the Streptomyces genus can be exploited in environmentally friendly approaches to food safety. Genome information can help to characterize bioactive strains opening the possibility to decipher their mechanisms of action. The biocontrol and plant growth-promoting activity of two Streptomyces spp. strains, DEF1AK and DEF147AK, were assessed in vitro and in planta. The genome sequences were determined using the Illumina NextSeq sequencing system and were assembled using EvoCAT (Evogene Clustering and Assembly Toolbox). Streptomyces spp. DEF1AK and DEF147AK were able to improve seed germination and early plant development of maize, wheat, and tomato and inhibited the mycelium growth of diverse fungal plant pathogens in vitro. The genome sequence analysis identified both strains as S. albidoflavus (99% sequence identity). Both genomes were of 7.1-Mb length with an average GC content of 73.45%. AntiSMASH and MIBiG analyses revealed strain-specific sets of secondary metabolite gene clusters in the two strains as well as differences in the number and type of duplicated genes. The combination of the biological activity and genomic data is the basis for in-depth studies aimed at the identification of secondary metabolites involved in plant growth-promoting and biocontrol activity of Streptomyces spp. The comparison of unique genomic features of the two strains will help to explain their diverse biocontrol and plant growth-promoting activities and warrant targeted functional genomics approaches to verify their mechanisms of action.

中文翻译：

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具有植物生长和生物防治潜力的两个链霉链霉菌DEF1AK和DEF147AK的基因组序列草案

属于链霉菌属的细菌可用于环保的食品安全方法中。基因组信息可以帮助表征生物活性菌株，从而为破译其作用机理提供了可能。两种链霉菌的生物防治和促进植物生长的活性。在体外和植物中评估了DEF1AK和DEF147AK菌株。使用Illumina NextSeq测序系统确定基因组序列，并使用EvoCAT（Evogene聚类和组装工具箱）进行组装。链霉菌 DEF1AK和DEF147AK能够改善玉米，小麦和番茄的种子发芽和早期植物发育，并在体外抑制多种真菌植物病原体的菌丝体生长。基因组序列分析将这两种菌株都鉴定为albidoflavus（99％序列同一性）。两个基因组的长度为7.1 Mb，平均GC含量为73.45％。AntiSMASH和MIBiG分析揭示了两个菌株中特定于菌株的次生代谢产物基因簇集，以及重复基因的数量和类型上的差异。生物活性和基因组数据的结合是深入研究的基础，旨在鉴定与链霉菌属植物的植物生长促进和生物防治活性有关的次生代谢产物。两种菌株独特的基因组特征的比较将有助于解释其多样化的生物防治和促进植物生长的活性，并有针对性的功能基因组学方法来验证其作用机理。AntiSMASH和MIBiG分析揭示了两种菌株中菌株特异性的次级代谢产物基因簇集，以及重复基因的数量和类型的差异。生物活性和基因组数据的结合是深入研究的基础，该研究旨在鉴定与链霉菌属植物的植物生长促进和生物防治活性有关的次生代谢产物。两种菌株独特基因组特征的比较将有助于解释其多样化的生物防治和促进植物生长的活性，并有针对性的功能基因组学方法来验证其作用机理。AntiSMASH和MIBiG分析揭示了两种菌株中菌株特异性的次级代谢产物基因簇集，以及重复基因的数量和类型的差异。生物活性和基因组数据的结合是深入研究的基础，该研究旨在鉴定与链霉菌属植物的植物生长促进和生物防治活性有关的次生代谢产物。两种菌株独特基因组特征的比较将有助于解释其多样化的生物防治和促进植物生长的活性，并有针对性的功能基因组学方法来验证其作用机理。生物活性和基因组数据的结合是深入研究的基础，旨在鉴定与链霉菌属植物的植物生长促进和生物防治活性有关的次生代谢产物。两种菌株独特的基因组特征的比较将有助于解释其多样化的生物防治和促进植物生长的活性，并有针对性的功能基因组学方法来验证其作用机理。生物活性和基因组数据的结合是深入研究的基础，该研究旨在鉴定与链霉菌属植物的植物生长促进和生物防治活性有关的次生代谢产物。两种菌株独特基因组特征的比较将有助于解释其多样化的生物防治和促进植物生长的活性，并有针对性的功能基因组学方法来验证其作用机理。