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Antioxidant Activity and Biocompatibility of Fructo-Polysaccharides Extracted from a Wild Species of Ornithogalum from Lebanon
Antioxidants ( IF 7 ) Pub Date : 2021-01-07 , DOI: 10.3390/antiox10010068
Mohammad Kazem Medlej , Cherri Batoul , Hamza Olleik , Suming Li , Akram Hijazi , Ghassan Nasser , Marc Maresca , Céline Pochat-Bohatier

The present study aims to investigate the properties of biopolymers extracted from a Lebanese onion non edible plant. The extraction was performed under mild conditions by varying the percentage of ultra-sound (US) treatment duration to a total extraction time of 30 min (0, 50, 100% US). The extracts were characterized using FTIR, SEC, GC-MS, TGA, and DSC analyses. The composition of the extracts was determined from the total carbohydrate content and protein content measurements. The thermal analyses indicate that all samples have high thermal stability. The antioxidant activities of the extracts were investigated, using β-carotene bleaching, scavenging activity of ABTS, metal chelating ability, and total antioxidant activity tests. The results indicate that the 50% US treatment leads to the best antioxidant activity. Biocompatibility of the extracts was evaluated using hemolysis and cytotoxicity assays. The results showed that 0 and 50% US samples are not toxic to human cells, in contrary to 100% US.

中文翻译:

从黎巴嫩野生虎眼草中提取的果糖多糖的抗氧化活性和生物相容性

本研究旨在调查从黎巴嫩洋葱非食用植物中提取的生物聚合物的特性。提取是在温和条件下进行的,方法是将超声处理持续时间的百分比更改为总提取时间为30分钟(0、50、100%US)。使用FTIR,SEC,GC-MS,TGA和DSC分析对提取物进行表征。从总碳水化合物含量和蛋白质含量测量值确定提取物的组成。热分析表明,所有样品均具有较高的热稳定性。使用β-胡萝卜素漂白,ABTS清除活性,金属螯合能力和总抗氧化活性测试,研究了提取物的抗氧化活性。结果表明,50%的US处理可产生最佳的抗氧化活性。使用溶血和细胞毒性试验评估提取物的生物相容性。结果表明,与100%US相反,0%和50%US样品对人细胞无毒。
更新日期:2021-01-07
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