Plant extracts have gained more attention as natural therapeutic agents against inflammation characterized by an overproduction of several inflammatory mediators such as reactive oxygen species and pro-inflammatory cytokines. Although Abeliophyllum distichum Nakai is generally known for its ornamental value, recent pharmacological research has demonstrated its potential therapeutic properties. Thus, to further evaluate the applicability of A. distichum in the food, cosmetic, and medical industries, we identified the phytochemicals in three organ extracts (fruits: AF, branches: AB, leaves: AL) of A. distichum and determined their antioxidant and anti-inflammatory activities. Using UPLC-ESI-Q-TOF-MS, a total of 19 compounds, including dendromoniliside D, forsythoside B, isoacteoside, isomucronulatol 7-O-Glucoside, plantamajoside, and wighteone were identified in the A. distichum organ extracts. AB exhibited a strong reducing power, an oxygen radical antioxidant capacity, and radical scavenging values compared with other samples, whereas AL exhibited the best anti-inflammatory properties. Gene expression, western blot, and molecular docking analyses suggested that the anti-inflammatory effect of AL was mediated by its ability to suppress lipopolysaccharide (LPS)-induced production of reactive oxygen species and/or inhibit LPS-stimulated activation of extracellular signal-regulated protein kinases (ERK1/2) in RAW264.7 cells. Collectively, these results indicate that AL is a potential source of phytochemicals that could be used to treat inflammation-associated diseases.

中文翻译：

---

基于UPLC-ESI-Q-TOF-MS的山茶不同器官提取物的代谢产物分析，抗氧化和抗炎特性

植物提取物作为抗炎的天然治疗剂受到了更多的关注，其特征在于几种炎性介质如活性氧和促炎细胞因子的过量生产。尽管中天茶（Abeliophyllum distichum Nakai）因其具有观赏价值而广为人知，但最近的药理研究表明其潜在的治疗特性。因此，为了进一步评价的适用性A.杉在食品，化妆品，医药等行业，我们确定了三个器官提取物（水果AL：AF，分支：AB，叶）的植物化学物质的一个。dist并确定了它们的抗氧化和消炎活性。使用UPLC-ESI-Q-TOF-MS，在dist.A. distichum中鉴定出总共19种化合物，包括树突莫尼糖苷D，邻苯二酚B，异乳糖苷，异核糖醛7-O-葡糖苷，车前苦甙和维特酮。器官提取物。与其他样品相比，AB表现出强大的还原力，氧自由基抗氧化能力和自由基清除值，而AL表现出最佳的抗炎特性。基因表达，蛋白质印迹和分子对接分析表明，AL的抗炎作用是由其抑制脂多糖（LPS）诱导产生的活性氧和/或抑制LPS刺激的细胞外信号调节激活的能力介导的RAW264.7细胞中的蛋白激酶（ERK1 / 2）。总体而言，这些结果表明，AL是可用于治疗炎症相关疾病的植物化学物质的潜在来源。