当前位置: X-MOL 学术Transl. Psychiaty › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
PET evaluation of light-induced modulation of microglial activation and GLP-1R expression in depressive rats
Translational Psychiatry ( IF 6.8 ) Pub Date : 2021-01-06 , DOI: 10.1038/s41398-020-01155-z
Yu Liu 1, 2 , Lizhen Wang 1 , Donghui Pan 1 , Mingzhu Li 1 , Yaoqi Li 1 , Yan Wang 1 , Yuping Xu 1 , Xinyu Wang 1 , Junjie Yan 1 , Qiong Wu 1 , Lin Lu 2, 3 , Kai Yuan 3 , Min Yang 1
Affiliation  

Light therapy has been accepted as a promising therapeutic choice for depression. Positron emission tomography (PET) combined with specific radiotracers has great benefits for revealing pathogenesis and developing therapeutics. This study aimed to investigate the influences of light therapy on microglial activation and glucagon-like peptide-1 receptor (GLP-1R) expression in the brain of depressive rats using [18F]DPA-714 and [18F]exendin-4 PET. The results showed that chronic unpredictable mild stress (CUMS)-induced depressive rats had poorer performance in behavioral tests compared to normal rats (p < 0.05) and the depressive-like behavior could be ameliorated by light therapy. Besides, depressive rats had significantly higher [18F]DPA-714 uptake and lower [18F]FDG uptake compare to normal rats in 11 and 9 regions of interest (ROIs) of the brain, respectively (p < 0.05). After 5 weeks of light therapy, higher [18F]FDG and [18F]exendin-4 uptake was observed in most ROIs of light therapy-treated depressive rats compared to untreated depressive rats (p < 0.05) and no significant differences existed in [18F]DPA-714 uptake between the two groups. This study demonstrated that light therapy can ameliorate depressive-like behavior, improve glucose metabolism, and halt the decline of brain GLP-1R expression of depressive rats, but have no effects on microglial activation caused by CUMS. Besides, this study validated that [18F]DPA-714 and [18F]exendin-4 PET have the potential for noninvasive evaluation of microglial activation and GLP-1R expression in the brain of depression.



中文翻译:

PET评估抑郁症大鼠光诱导的小胶质细胞活化和GLP-1R表达的调节

光疗法已被接受为抑郁症的有前途的治疗选择。正电子发射断层扫描(PET)结合特定的放射性示踪剂在揭示发病机理和开发治疗方法方面具有巨大优势。这项研究旨在研究使用[ 18 F] DPA-714和[ 18 F] exendin-4 PET对光疗法对抑郁大鼠脑中小胶质细胞活化和胰高血糖素样肽1受体(GLP-1R)表达的影响。。结果表明,与正常大鼠相比,慢性不可预测的轻度应激(CUMS)诱发的抑郁大鼠在行为测试中的表现较差(p  <0.05),并且通过光疗可以改善类似抑郁的行为。此外,抑郁症大鼠的血压明显升高[ 18与正常大鼠相比,在大脑的11个和9个感兴趣区域(ROI)中,F] DPA-714的摄取量和更低的[ 18 F] FDG摄取量均较低(p  <0.05)。在光疗5周后,与未治疗的抑郁大鼠相比,在光疗治疗的抑郁大鼠的大多数ROI中观察到更高的[ 18 F] FDG和[ 18 F] exendin-4摄取(p  <0.05),并且[ 18两组之间的F] DPA-714摄取。这项研究表明,光疗可以改善抑郁症样行为,改善葡萄糖代谢,阻止抑郁症大鼠脑GLP-1R表达的下降,但对CUMS引起的小胶质细胞活化没有影响。此外,这项研究证实了[ 18 F] DPA-714和[ 18 F] exendin-4 PET具有潜在性地评估抑郁症大脑中小胶质细胞激活和GLP-1R表达的潜力。

更新日期:2021-01-07
down
wechat
bug