Active matrix metalloproteinase-8 and interleukin-6 detect periodontal degeneration caused by radiotherapy of head and neck cancer: a pilot study,Expert Review of Proteomics

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Active matrix metalloproteinase-8 and interleukin-6 detect periodontal degeneration caused by radiotherapy of head and neck cancer: a pilot study
Expert Review of Proteomics ( IF 3.4 ) Pub Date : 2021-01-07 , DOI: 10.1080/14789450.2020.1858056
Mutlu Keskin ₁ , Hanna Lähteenmäki ₂ , Nilminie Rathnayake ₂ , Ismo T Räisänen ₂ , Taina Tervahartiala ₂ , Pirjo Pärnänen ₂ , Ahmet Murat Şenışık ₃ , Didem Karaçetin ₄ , Ayben Yentek Balkanay ₄ , Pia Heikkilä ₂ , Jaana Hagström _{5,

6,

7} , Jaana Rautava _{2,

5,

8} , Caj Haglund _{7,

9} , Ulvi Kahraman Gursoy ₁₀ , Angelika Silbereisen ₁₁ , Nagihan Bostanci ₁₁ , Timo Sorsa _{2,

11}

Affiliation

Oral and Dental Health Department, Altınbaş University , İstanbul, Turkey.
Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki University Hospital , Helsinki, Finland.
Radiotherapy Department, Altınbaş University , İstanbul, Turkey.
Radiation Oncology Department, Başakşehir Çam and Sakura City Hospital , İstanbul, Turkey.
Department of Oral Pathology and Radiology, University of Turku , Turku, Finland.
Department of Pathology HUSLAB, Helsinki University Hospital , Helsinki, Finland.
Research Programs Unit, Translational Cancer Medicine, University of Helsinki , Helsinki, Finland.
Department of Pathology, HUSLAB & University of Helsinki , Helsinki, Finland.
Department of Surgery, University of Helsinki and Helsinki University Hospital , Helsinki, Finland.
Department of Periodontology, Institute of Dentistry, University of Turku , Turku, Finland.
Section of Periodontology and Dental Prevention, Division of Oral Diseases, Department of Dental Medicine , Karolinska Institutet, Sweden.

ABSTRACT

Background: This cohort study investigated the role of the active matrix metalloproteinase-8 (aMMP-8) and interleukin-6 (IL-6) as oral fluid biomarkers for monitoring the periodontal degeneration occurring in head and neck cancer (HNC) patients treated by radiotherapy.

Research design and methods: Eleven patients, aged 28–74, diagnosed with HNC were included in the study. Complete periodontal and oral examinations were performed pre-radiotherapy and 1 month after radiotherapy. Mouthrinse samples (pre-radiotherapy, after 6 weeks of radiotherapy and 1 month after radiotherapy) were assayed by aMMP-8 point-of-care-kit (PerioSafe®/ORALyzer®) for aMMP-8 and ELISA for IL-6.

Results: HNC radiotherapy had a deteriorating impact on the periodontium and a significant impact on periodontal biomarkers aMMP-8 and IL-6 and increased their levels in mouthrinse. Clinical-attachment-loss (CAL) (site of greatest loss: mean = 1.7 mm, range = 1–3 mm) corresponding to rapid progression of periodontitis. There was a positive repeated measures correlation (rmcorr = 0.667) between the aMMP-8 and IL-6 levels.

Conclusions: Elevated aMMP-8 levels were observed 1 month after radiotherapy among some HNC patients suggesting a prolonged increased susceptibility to further periodontal tissue destruction. Currently available aMMP-8 point-of-care testing could be useful to monitor and assess quantitatively online and real-time the risk of deterioration of periodontal health during HNC radiotherapy.

中文翻译：

活性基质金属蛋白酶 8 和白细胞介素 6 检测头颈癌放疗引起的牙周变性：一项初步研究

摘要

背景：这项队列研究调查了活性基质金属蛋白酶 8 (aMMP-8) 和白细胞介素 6 (IL-6) 作为口腔液生物标志物的作用，用于监测头颈癌 (HNC) 治疗的头颈癌 (HNC) 患者发生的牙周变性。放疗。

研究设计和方法：研究包括11 名年龄在 28-74 岁之间的被诊断患有 HNC 的患者。在放疗前和放疗后 1 个月进行完整的牙周和口腔检查。漱口水样品（放疗前、放疗后 6 周和放疗后 1 个月）通过 aMMP-8 即时检测试剂盒 (PerioSafe®/ORALyzer®) 检测 aMMP-8 和 ELISA 检测 IL-6。

结果：HNC 放疗对牙周组织有恶化影响，对牙周生物标志物 aMMP-8 和 IL-6 有显着影响，并增加了它们在漱口水中的水平。临床附着丧失 (CAL)（最大丧失部位：平均值 = 1.7 毫米，范围 = 1-3 毫米）对应于牙周炎的快速进展。aMMP-8 和 IL-6 水平之间存在正的重复测量相关性 (rmcorr = 0.667)。

结论：在一些 HNC 患者放疗后 1 个月观察到 aMMP-8 水平升高，表明对进一步牙周组织破坏的易感性持续增加。目前可用的 aMMP-8 床旁检测可用于在线和实时定量监测和评估 HNC 放射治疗期间牙周健康恶化的风险。

更新日期：2021-02-09

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