Extracellular vesicles (EVs), which are nanosized vesicles released by cells as intracellular messengers, have high potential as biomarkers. EVs are usually collected from in vitro sources, such as cell culture media or biofluids, and not from tissues. Techniques enabling direct collection of EVs from tissues will extend the applications of EVs. We compared methods for separating EVs from solid liver, heart, and skeletal muscle. Compared with a precipitation method, an ultracentrifugation‐based method for collection of EVs from solid tissues yielded a higher proportion of EVs positive for EV‐related markers, with minimum levels of intracellular organelle‐related markers. Some tissue‐specific modifications, such as a sucrose cushion step, may improve the yield and purity of the collected EVs.

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肝脏、心脏和骨骼肌中组织来源的细胞外囊泡分离方法的比较

细胞外囊泡 (EVs) 是由细胞作为细胞内信使释放的纳米级囊泡，具有很高的生物标志物潜力。EVs 通常是从体外来源收集的，例如细胞培养基或生物流体，而不是从组织中收集。能够从组织中直接收集 EV 的技术将扩展 EV 的应用。我们比较了将 EV 从固体肝脏、心脏和骨骼肌中分离出来的方法。与沉淀法相比，基于超速离心的方法从实体组织中收集 EV 的 EV 相关标记物阳性比例更高，而细胞内细胞器相关标记物水平最低。一些组织特异性修饰，例如蔗糖缓冲步骤，可能会提高收集到的 EV 的产量和纯度。