A 60‐day feeding trial was conducted to evaluate growth response, digestive enzyme activities, digestibility and amylase gene expression of Labeo rohita fingerling fed with Siam weed (Chromolaena odorata) meal (SWM) by gradually replacing de‐oiled rice bran (DORB). One hundred and eighty fingerlings were randomly distributed into four distinct experimental groups in triplicates. Four semi‐purified iso‐nitrogenous diets (330 g kg⁻¹ CP) with different combinations of SWM and DORB such as T1 (diet with 300 g kg⁻¹ DORB + 0.0 g kg⁻¹ SWM), T2 (diet with 200.0 g kg⁻¹ DORB + 100.0 g kg⁻¹ SWM), T3 (diet with 100.0 g kg⁻¹ DORB + 200.0 g kg⁻¹ SWM) and T4 (diet with 0.0 g kg⁻¹ DORB +300.0 g kg⁻¹ SWM) were prepared and fed to satiation level. Among the various groups, T4 showed a significant reduction (p < 0.05) in feed intake than the other experimental groups. T3 group exhibited highest (p < 0.05) WG, SGR, PER compared to T1, T2 and T4 groups. Significantly higher (p < 0.05) amylase, protease, trypsin activities and amylase mRNA expression while lower chymotrypsin activity were recorded in the T3 group compared to the control group. In vitro and in vivo digestibility and metabolic enzyme activities were significantly higher (p < 0.05) for T3 diet fed fish compared to other groups. Overall results revealed that SWM can effectively include up to 300.0 g kg⁻¹ by successfully replacing equal amount of DORB without compromising the growth. Hence, it could be used as an alternate ingredient in the diet of Labeo rohita fingerlings.

中文翻译：

---

暹罗杂草粉（Chromolaena odorata）粉作为罗汉果日粮中DORB的消化促进剂和替代物：对生长，消化酶和淀粉酶基因表达的影响

60天饲养试验以评估生长反应，消化酶活性，消化率和淀粉酶基因表达南亚野鲮鱼种馈送暹杂草（飞机草）粉（SWM）通过逐步取代脱油的米糠（DORB）。将一百零八种鱼种一式三份随机分配到四个不同的实验组中。四种半精制的等氮饲料（330 g kg ^-1 CP），具有不同的SWM和DORB组合，例如T1（饮食为300 g kg ^-1 DORB + 0.0 g kg ^-1 SWM），T2（饮食为200.0 g千克^-1 DORB + 100.0千克kg ^-1 SWM），T3（饮食为100.0千克kg ^-1千克DORB + 200.0千克kg制备T 1（SWM ^-1）和T4（0.0g kg ^-1 DORB +300.0 g kg ^-1 SWM的饮食）并进料至饱食水平。在各个组中，T4的 采食量显着低于其他实验组（p <0.05）。 与T1，T2和T4组相比，T3组表现出最高（p <0.05）WG，SGR，PER。 与对照组相比，T3组的淀粉酶，蛋白酶，胰蛋白酶活性和淀粉酶mRNA表达明显较高（p <0.05），而胰凝乳蛋白酶活性较低。体外和体内的消化率和代谢酶活性显着较高（p 与其他组相比，T3饮食喂养的鱼<0.05）。总体结果表明，通过成功替代等量的DORB ，SWM可以有效地添加多达300.0 g kg ^-1而不损害生长。因此，它可以作为Labeo rohita鱼种饮食中的替代成分。