Broomrapes are important parasitic weeds in many vegetable crops in dry and warm regions of the world. The study of weed parasite physiology will increase knowledge of broomrape‐host interactions that may be applied to the development of improved parasitic plant control programmes. In this study, developmental patterns of activities of essential enzymes (sucrose synthase [SUS], mannose 6‐phosphate reductase [M6PR], cell wall invertase [CWI] and soluble acid invertase [SAI]) involved in Egyptian broomrape osmoregulation were investigated using three hosts (celery, tomato and cucumber). The earliest broomrape emergence occurred on cucumber; however, the total biomass of the parasite did not differ between cucumber and tomato. The reducing sugars content, gene expression and enzyme activity in the parasite significantly varied with the parasite organs and the hosts. Maximum SAI activity was observed in the apical part (AP) of the flowering shoot of parasite growing on cucumber. However, the peak of genes expression occurred in the same organ across the three hosts, for example SAI1 was expressed maximally in tubercle bearing the flowering shoot (Tub‐FS). The largest amount of reducing sugars was observed in the AP of the parasite grown on tomato. High M6PR activity in the parasite and its matching with M6PR transcriptional levels in three hosts are deemed promising for broomrape management through the deployment of the proteinaceous inhibitors or gene silencing constructs in host plants. Overall, the results indicated that differences in sugar metabolism of Egyptian broomrape grown on different hosts can potentially be exploited for effective management of this difficult‐to‐control parasite.

中文翻译：

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埃及broomrape蔗糖代谢对不同寄主植物的反应

扫帚草是世界上干燥和温暖地区许多蔬菜作物中重要的寄生杂草。对杂草寄生虫生理学的研究将增加扫帚-宿主相互作用的知识，这些知识可用于开发改进的寄生植物控制计划。在这项研究中，使用三种方法研究了参与埃及扫帚渗透调节的必需酶（蔗糖合酶[SUS]，6-磷酸甘露糖6-磷酸还原酶[M6PR]，细胞壁转化酶[CWI]和可溶性酸转化酶[SAI]）活性的发育模式。寄主（芹菜，西红柿和黄瓜）。最早的扫帚发芽出现在黄瓜上。但是，黄瓜和番茄之间的寄生虫总生物量没有差异。还原糖含量 寄生虫中的基因表达和酶活性随寄生虫器官和宿主而显着变化。在黄瓜上生长的寄生虫的花梢的顶端（AP）中观察到最大的SAI活性。但是，例如，基因表达的峰值出现在三个宿主的同一器官中SAI1在带有开花芽的结节中最大表达（Tub-FS）。在番茄上生长的寄生虫的AP中观察到最大量的还原糖。通过在宿主植物中部署蛋白质抑制剂或基因沉默构建体，该寄生虫中的高M6PR活性及其与3个宿主中M6PR转录水平的匹配被认为有望用于扫帚治疗。总体而言，结果表明，在不同宿主上生长的埃及扫帚糖在糖代谢上的差异可潜在地用于有效控制这种难以控制的寄生虫。