Asian Citrus Psyllid (ACP), Diaphorina citri, is a key vector transmitting the causative agent of Huanglongbing (HLB) disease. Population growth of ACP is evident after feeding on plant flush shoots, as they only oviposit here. However, the underlying mechanism as to why flush shoots govern oviposition is unclear. This study compares the fecundity and ovarian morphology of ACP between young flush and mature leaves. Furthermore, the transcriptome of mated females infesting Murraya paniculata was analysed. Finally, the gene of the key Target of Rapamycin (TOR) signalling pathway was silenced by RNAi. Results indicated that flush shoot feeding activated the development of the psyllids ovary and therefore induced oviposition. A total of 126 and 2794 differentially expressed genes were detected at 1 and 5 days, respectively, after pest infestation of flush shoots compared to mature leaves. Many genes are involved in protein metabolism, Mitogen‐Activated Protein Kinase (MAPK) signalling pathway, hormone synthesis, and TOR signalling pathway: all thought to activate reproduction. Silencing of the positive regulator gene DcRheb in the TOR pathway resulted in lower levels of ecdysone and juvenile hormone and decreased vitellogenin synthesis, further disrupting reproductive ability. This study enhances understanding of the molecular mechanism underlying ACP's reproductive strategy.

中文翻译：

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转录组分析揭示了 TOR 信号介导的植物红芽控制柑橘木瓜产卵

亚洲柑橘木虱 (ACP)，柑橘木虱，是传播黄龙病 (HLB) 病病原体的关键载体。ACP 的种群增长在以植物冲洗的枝条为食后很明显，因为它们只在这里产卵。然而，关于为什么红芽支配产卵的潜在机制尚不清楚。本研究比较了嫩叶和成熟叶之间 ACP 的繁殖力和卵巢形态。此外，感染Murraya paniculata的交配雌性的转录组进行了分析。最后，雷帕霉素 (TOR) 信号通路的关键目标基因被 RNAi 沉默。结果表明，冲洗枝条摄食激活木虱子房的发育并因此诱导产卵。与成熟叶片相比，在害虫侵染后的第 1 天和第 5 天，总共检测到 126 和 2794 个差异表达基因。许多基因参与蛋白质代谢、丝裂原活化蛋白激酶 (MAPK) 信号通路、激素合成和 TOR 信号通路：所有这些都被认为可以激活繁殖。正调节基因DcRheb 的沉默在 TOR 通路中导致蜕皮激素和保幼激素水平降低，卵黄蛋白原合成减少，进一步破坏生殖能力。这项研究增进了对 ACP 生殖策略背后的分子机制的理解。