Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has spread rapidly throughout the whole world and caused significant difficulties in the prevention and control of the epidemic. In this case, several detection methods have been established based on nucleic acid diagnostic techniques and immunoassays to achieve sensitive and specific detection of SARS‐CoV‐2. However, most methods are still largely dependent on professional instruments, highly trained operators, and centralized laboratories. These limitations gravely diminish their practicality and portability. Herein, a clustered regularly interspaced short palindromic repeats (CRISPR) Cas12a based assay was developed for portable, rapid and sensitive of SARS‐CoV‐2. In this assay, samples were quickly pretreated and amplified by reverse transcription recombinase‐aided amplification under mild conditions. Then, by combining the CRISPR Cas12a system and a glucose‐producing reaction, the signal of the virus was converted to that of glucose, which can be quantitatively read by a personal glucose meter in a few seconds. Nucleocapsid protein gene was tested as a model target, and the sensitivity for quantitative detection was as low as 10 copies/μl, which basically meet the needs of clinical diagnosis. In addition, with the advantages of lower material cost, shorter detection time, and no requirement for professional instrument in comparison with quantitative reverse transcription‐polymerase chain reaction, this assay is expected to provide a powerful technical support for the early diagnosis and intervention during epidemic prevention and control.

中文翻译：

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源自CRISPR-Cas12a的生物传感器，支持便携式个人血糖仪读数以定量检测SARS-CoV-2

严重的急性呼吸系统综合症冠状病毒2（SARS-CoV-2）在全世界迅速传播，并在预防和控制该流行病方面造成重大困难。在这种情况下，已经建立了几种基于核酸诊断技术和免疫测定的检测方法，以实现对SARS-CoV-2的灵敏和特异性检测。但是，大多数方法仍然很大程度上取决于专业仪器，训练有素的操作员和集中实验室。这些限制严重削弱了它们的实用性和可移植性。在本文中，开发了一种基于聚簇的规则间隔的短回文重复序列（CRISPR）Cas12a的分析方法，可用于SARS-CoV-2的便携式，快速和灵敏。在这个实验中 快速样品预处理，并在温和条件下通过逆转录重组酶辅助扩增进行扩增。然后，通过将CRISPR Cas12a系统和产生葡萄糖的反应结合起来，病毒的信号被转换为葡萄糖的信号，这可以由个人血糖仪在几秒钟内定量读取。以核壳蛋白基因为模型靶标，定量检测的灵敏度低至10拷贝/μl，基本满足临床诊断的需要。此外，与定量逆转录聚合酶链反应相比，具有材料成本低，检测时间短以及不需要专业仪器的优势，