Dioecy, the presence of male and female individual, has evolved independently from hermaphroditism in multiple flowering plant lineages. Spinach, an important leafy vegetable crop worldwide, is a dioecious species with an XY sex-determination system. Although some markers that are fully linked to male-determination locus were identified, the male-specific region (MSR) remained unclear. In this research, five male individuals and five female lines were resequenced to identify the male-specific region. We identified a region (∼21 kb) on chromosome 4 (putative sex chromosome) where the five females had a low reads coverage, while the five males had high coverage. A KASP marker, SponR, developed from a single nucleotide polymorphism (SNP) closely linked to the MSR was co-segregating with the sex determination gene in the population of 958 individuals, suggesting that the MSR might be specifically present in male spinach plants.

Dioecy，男性和女性个体的存在，在多个开花植物谱系中独立于雌雄同体进化。菠菜是世界范围内重要的多叶蔬菜作物，是一种具有 XY 性别决定系统的雌雄异株物种。尽管确定了一些与雄性决定基因座完全相关的标记，但雄性特异性区域 (MSR) 仍不清楚。在这项研究中，对五个雄性个体和五个雌性系进行了重新测序，以确定雄性特异性区域。我们在 4 号染色体（假定的性染色体）上确定了一个区域（~21 kb），其中五名女性的读数覆盖率较低，而五名男性的读数覆盖率较高。从与 MSR 密切相关的单核苷酸多态性 (SNP) 发展而来的 KASP 标记 SponR 与 958 个人中的性别决定基因共同分离，