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dmrt11E ortholog is a crucial factor for oogenesis of the domesticated silkworm, Bombyx mori
Insect Biochemistry and Molecular Biology ( IF 3.8 ) Pub Date : 2021-01-07 , DOI: 10.1016/j.ibmb.2020.103517
Ryota Kasahara 1 , Tomohisa Yuzawa 2 , Takehsi Fujii 3 , Fugaku Aoki 1 , Masataka G Suzuki 1
Affiliation  

DMRT (Doublesex and Mab-3-related transcription factor) is a highly conserved transcription factor family involved in sex determination in numerous animal species. One DMRT, dmrt2/dmrt11E, has entirely different functions in invertebrate and vertebrate species, indicating unpredicted functions. Here, we performed functional analysis of the dmrt11E gene in the domesticated silkworm, Bombyx mori. This gene was preferentially expressed in ovarioles at the last larval instar stage. Its mRNA accumulated in ovarian eggs during the adult stage. CRISPR/Cas9-mediated knockout of Bombyx dmrt11E (Bmdmrt11E) caused defects in oogenesis, resulting in the production of abnormal eggs with transparent liquids. These eggs had significantly reduced fertility and lipid levels. Transcriptomic comparisons between ovaries of control and mutant insects at two developmental stages identified six genes that may be under the control of Bmdmrt11E. Finally, we provide a possible model for lipid uptake and storage in eggs of Bombyx mori.



中文翻译:

dmrt11E直系同源物是家蚕Bombyx mori发生的关键因素

DMRT(Doublesex和与Mab-3相关的转录因子)是高度保守的转录因子家族,参与许多动物物种的性别确定。一种DMRT,即dmrt2 / dmrt11E,在无脊椎动物和脊椎动物中具有完全不同的功能,表明功能是不可预测的。在这里,我们对家蚕Bombyx mori中的dmrt11E基因进行了功能分析。该基因在幼虫的最后一个成年阶段优先在卵巢中表达。成年期其mRNA在卵巢卵中积累。CRISPR / Cas9介导的Bombyx dmrt11EBmdmrt11E的敲除)导致卵子发生缺陷,导致产生带有透明液体的异常卵。这些卵的生育力和血脂水平明显降低。在两个发育阶段的对照和突变昆虫卵巢之间的转录组比较,鉴定了可能在Bmdmrt11E控制下的六个基因。最后,我们提供了家蚕卵中脂质吸收和储存的可能模型。

更新日期:2021-01-10
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