Dendropanax morbifera extracts were obtained from three different tissues: the heartwood, stem bark, and leaves. The 95% ethanol extract from leaf showed the strongest inhibitory activity (88% inhibition at 50 μg/mL with HepG2 cells) against triglyceride (TG) biosynthesis. Bioactivity-guided fractionation and metabolite investigation of the leaf extracts resulted in the separation and identification of three polyacetylene derivatives that inhibited newly synthesized TG in HepG2 cells. A method for the profile and contents analyses of the leaf extracts were measured using QTOF-MS with a charged aerosol detector (CAD), including (9Z,16S)-16-hydroxy-9,17-octadecadiene-12,14-diynoic acid (1), which was decomposed during the temperature and storage studies. All metabolites decomposed at different temperatures, especially the polyacetylene, which was rapidly oxidized when the isolated form was exposed to air. However, it was fairly stable in the extract at low temperature <–20 °C. The aim of this study was to provide a way to protect the active ingredients in the process of developing and distributing functional foods containing D. morbifera. The application of this quality control/quality assurance step may improve the chemistry, manufacturing, and control for raw materials.

Dendropanax morbifera提取物是从三种不同的组织中获得的：心材，茎皮和叶子。从叶中提取的95％乙醇提取物对甘油三酸酯（TG）的生物合成表现出最强的抑制活性（HepG2细胞在50μg/ mL时抑制率达88％）。叶提取物的生物活性指导分级分离和代谢产物研究导致分离和鉴定了三种抑制HepG2细胞中新合成的TG的聚乙炔衍生物。使用带电荷气溶胶检测器（CAD）的QTOF-MS测量了叶提取物的特征和含量的方法，该方法包括（9 Z，16 S）-16-羟基-9,17-十八碳二烯-12,14-二壬酸（1），在温度和储存条件下会分解。所有代谢物在不同温度下分解，尤其是聚乙炔，当分离的形式暴露于空气中时，乙炔会迅速氧化。但是，它在<-20°C的低温下在提取物中相当稳定。这项研究的目的是提供一种在开发和分发含D. morbifera功能食品的过程中保护有效成分的方法。此质量控制/质量保证步骤的应用可以改善原材料的化学，制造和控制。