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Kinetic study of activation and deactivation of adsorbed cellulase during enzymatic conversion of alkaline peroxide oxidation-pretreated corn cob to sugar
Korean Journal of Chemical Engineering ( IF 2.7 ) Pub Date : 2021-01-01 , DOI: 10.1007/s11814-020-0667-2
Augustine Omoniyi Ayeni , Oluranti Agboola , Michael Olawale Daramola , Bianca Grabner , Babalola Aisosa Oni , Damilola Elizabeth Babatunde , Joseph Evwodere

Corn cob lignocellulosic biomass is one of the useful precursors for the alternative production of fuels and chemicals. Understanding the kinetics of enzymatic conversion of corn cob through kinetic models could provide indepth knowledge and increase the predictive ability for process design and optimization. In this study, models based on the semi-mechanistic rate equations, first-order decay exponential function of time for adsorbed enzymes, structural and diffusion coefficient for adsorption were used to estimate kinetic parameters for the enzymatic conversion of alkaline peroxide oxidation (APO) pretreated corn cob to sugar. Fitting a first-order inactivation model of adsorbed cellulases to account for experimental hydrolysis data, the apparent hydrolysis rate constant (k 2 =29.51 min −1 ), the inactivation rate constant (k 3 =0.269 min −1 ), and reactivation rate constant (k 4 =0.0048 min −1 ) were estimated. Regressed values of apparent maximum rate, V max, app , for adsorbed enzymes reduced appreciably with time to more than 98% at 96 h. The diffusion limit model showed that the diffusion resistance increased with increasing enzyme concentrations.

中文翻译:

碱性过氧化物氧化预处理玉米芯转化糖过程中吸附纤维素酶活化和失活的动力学研究

玉米芯木质纤维素生物质是替代生产燃料和化学品的有用前体之一。通过动力学模型了解玉米芯的酶促转化动力学可以提供深入的知识并提高工艺设计和优化的预测能力。在这项研究中,基于半机械速率方程、吸附酶的一阶衰减指数函数、吸附的结构和扩散系数的模型被用来估计碱性过氧化物氧化 (APO) 预处理的酶促转化的动力学参数玉米芯加糖。拟合吸附纤维素酶的一阶失活模型以解释实验水解数据、表观水解速率常数 (k 2 =29.51 min -1 )、失活速率常数 (k 3 =0.269 min -1 ),和再活化速率常数(k 4 =0.0048 min -1 )被估计。吸附酶的表观最大速率、V max 和 app 的回归值随时间显着降低至 96 小时时的 98% 以上。扩散极限模型表明,扩散阻力随着酶浓度的增加而增加。
更新日期:2021-01-01
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