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Temporal expression profiles of defense-related genes involved in Lactuca sativa- Sclerotinia sclerotiorum interactions
Journal of Plant Pathology ( IF 2.2 ) Pub Date : 2021-01-07 , DOI: 10.1007/s42161-020-00714-z Aqeel Hussein Abdulraoof Almatwari , Mohammadreza Hassandokht , Frouzandeh Soltani , Amir Mirzadi Gohari , Mohammad Javan-Nikkhah
Journal of Plant Pathology ( IF 2.2 ) Pub Date : 2021-01-07 , DOI: 10.1007/s42161-020-00714-z Aqeel Hussein Abdulraoof Almatwari , Mohammadreza Hassandokht , Frouzandeh Soltani , Amir Mirzadi Gohari , Mohammad Javan-Nikkhah
Sclerotinia sclerotiorum , a destructive fungal pathogen with an extensive host range infecting more than 400 plant species, causes lettuce drop on the leafy green lettuce that potentially have an enormous economic impact on lettuce cultivation worldwide. To gain insights into how lettuce regulates its defense pathways, gene expression profiles of five defense-related genes ( LsPRB1 , LsSOD , LsERF1 , LsLTC1 , and LsHPL1 ) triggered following infection of susceptible Mazandaran line 1 (ML1) and tolerant Jahrom (Jah) lettuce accessions by the S. sclerotiorum were compared by the real-time quantitative RT-PCR (RT-qPCR) approach. In the current study, we observed temporal and quantitative gene expression fluctuations between two examined accessions of L. sativa in response to S. sclerotiorum attack. All genes, except LsHPL1 , were up-regulated earlier (24 hours after inoculation) in the Jah accession compared with the susceptible one. This data implies strong defense responses established in the tolerant accession to arrest the fungal growth, but it resulted in restricting lesion development rather than in preventing infection. This research contributes to a better understanding of the kinetics of lettuce reactions induced following S. sclerotiorum infection and may be employed to develop effective strategies to manage lettuce drop.
中文翻译:
参与莴苣-核盘菌相互作用的防御相关基因的时间表达谱
核盘菌是一种破坏性真菌病原体,宿主范围广泛,可感染 400 多种植物物种,导致绿叶生菜上的生菜掉落,这可能对全球生菜种植产生巨大的经济影响。为了深入了解生菜如何调节其防御途径,五个防御相关基因(LsPRB1、LsSOD、LsERF1、LsLTC1 和 LsHPL1)的基因表达谱在易感 Mazandaran 线 1 (ML1) 和耐受 Jahrom (Jah) 生菜感染后触发通过实时定量 RT-PCR (RT-qPCR) 方法比较了核盘菌的种质。在当前的研究中,我们观察到两个受检的 L. sativa 种质之间的时间和数量基因表达波动,以响应 S. sclerotiorum 攻击。所有基因,除了 LsHPL1 ,与易感种质相比,在 Jah 种质中更早(接种后 24 小时)上调。该数据意味着在耐受性加入中建立了强大的防御反应以阻止真菌生长,但它导致限制病变发展而不是预防感染。这项研究有助于更好地了解核盘菌感染后诱导的生菜反应动力学,并可用于制定有效的策略来管理生菜掉落。
更新日期:2021-01-07
中文翻译:
参与莴苣-核盘菌相互作用的防御相关基因的时间表达谱
核盘菌是一种破坏性真菌病原体,宿主范围广泛,可感染 400 多种植物物种,导致绿叶生菜上的生菜掉落,这可能对全球生菜种植产生巨大的经济影响。为了深入了解生菜如何调节其防御途径,五个防御相关基因(LsPRB1、LsSOD、LsERF1、LsLTC1 和 LsHPL1)的基因表达谱在易感 Mazandaran 线 1 (ML1) 和耐受 Jahrom (Jah) 生菜感染后触发通过实时定量 RT-PCR (RT-qPCR) 方法比较了核盘菌的种质。在当前的研究中,我们观察到两个受检的 L. sativa 种质之间的时间和数量基因表达波动,以响应 S. sclerotiorum 攻击。所有基因,除了 LsHPL1 ,与易感种质相比,在 Jah 种质中更早(接种后 24 小时)上调。该数据意味着在耐受性加入中建立了强大的防御反应以阻止真菌生长,但它导致限制病变发展而不是预防感染。这项研究有助于更好地了解核盘菌感染后诱导的生菜反应动力学,并可用于制定有效的策略来管理生菜掉落。