For transforming the massive amounts of extensive wasted glycerol from the procedure of biodiesels production, oleaginous yeasts were isolated from an activated sludge tank located at a biodiesel manufacturing factory in this study. One of the isolated oleaginous yeast proved with the most lipid production potential was identified as Rhodotorula mucilaginosa LP-2 by 18S rDNA sequencing. The optimal conditions for lipid production among strain R. mucilaginosa LP-2 were investigated. The optimum temperature for maximum lipid productivity was 33 °C. The most suitable carbon and nitrogen sources for strain R. mucilaginosa LP-2 to accumulate lipids were pure glycerol and yeast extract, respectively. C/N ratio was also demonstrated to be the significant factor for intracellular lipid accumulation. Cell growth and lipid production were both inhibited when the C/N ratio was higher than 85. The highest lipid yield was observed at C/N ratio of 65 due to a significant impact on the degree of fatty acid desaturation of this strain.

为了从生物柴油生产过程中转化大量的大量浪费的甘油，从位于本研究中的生物柴油制造工厂的活性污泥罐中分离出油质酵母。通过18S rDNA测序鉴定出一种分离的产油酵母，其具有最大的脂质生产潜力，被鉴定为粘液红假单胞菌LP-2。研究了粘菌R. mucilaginosa LP-2中脂质产生的最佳条件。最大脂质生产率的最佳温度为33°C。最适合粘菌R. mucilaginosa的碳和氮源LP-2积累脂质分别是纯甘油和酵母提取物。C / N比也被证明是细胞内脂质积累的重要因素。当C / N比高于85时，细胞的生长和脂质的产生都受到抑制。在65的C / N比下，观察到最高的脂质产量，这是由于对该菌株的脂肪酸去饱和度有重大影响。