Transglutaminase (TG) catalyzes the formation of cross-links between proteins. TG from Streptoverticillium mobaraense (SmTG) is used widely in food, cosmetic, biomaterial and medical industries. SmTG is occasionally supplied as a mixture with the activator peptide glutathione. Currently, glutathione is industrially produced using a budding yeast, Saccharomyces cerevisiae, because of its intracellular high content of glutathione. In this study, active SmTG was produced together with glutathione in S. cerevisiae. SmTG extracted from S. cerevisiae expressing SmTG showed cross-linking activity when BSA and sodium caseinate were substrates. The cross-linking activity of SmTG increased proportionally as the concentration of added glutathione increased. Furthermore, SmTG was prepared by extracting SmTG from an engineered S. cerevisiae whose glutathione synthetic pathway was enhanced. The SmTG solution showed higher activity when compared with a SmTG solution prepared from a S. cerevisiae strain without enhanced glutathione production. This result indicates that a high content of intracellular glutathione further enhances active SmTG production in S. cerevisiae. S. cerevisiae co-producing SmTG and a higher content of glutathione has the potential to supply a ready-to-use industrial active TG solution.

转谷氨酰胺酶（TG）催化蛋白质之间交联的形成。Mobaraense链霉菌的TG （Sm TG）广泛用于食品，化妆品，生物材料和医疗行业。Sm TG有时与激活肽谷胱甘肽以混合物形式提供。当前，由于谷胱甘肽在细胞内高含量的谷胱甘肽，因此工业上使用发芽酵母酿酒酵母（Saccharomyces cerevisiae）生产。在这项研究中，啤酒酵母中产生了活性Sm TG和谷胱甘肽。从表达Sm的酿酒酵母提取的Sm TG当BSA和酪蛋白酸钠为底物时，TG显示出交联活性。Sm TG的交联活性随所添加的谷胱甘肽浓度的增加而成比例地增加。此外，钐TG被提取制备的Sm从经工程改造的TG酿酒酵母，其谷胱甘肽合成途径增强。所述的Sm当与TG相比溶液显示更高的活性的Sm从制备TG溶液酿酒酵母而不增强谷胱甘肽生产菌株。该结果表明，高含量的细胞内谷胱甘肽进一步增强了酿酒酵母中活性Sm TG的产生。啤酒酵母联合生产Sm TG和更高含量的谷胱甘肽具有提供现成的工业活性TG溶液的潜力。