Virus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which are similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed against Senecavirus A (SVA). Our assay utilizes SVA VLPs that were expressed and assembled in an E. coli expression system as the coating antigens. VLPs have better safety and immunogenicity than intact viral particles or peptides. The VLPs-based cELISA was used to test 342 serum samples collected from different pig farms, and the results showed that its specificity and sensitivity were 100% and 94%, respectively. The consistency rates of cELISA with the BIOSTONE AsurDx™ Senecavirus A (SVA) Antibody Test Kit and an indirect immunofluorescent assay were 90.0% and 94.2%, respectively. Therefore, this VLPs-based cELISA can be effectively and reliably used for the detection and discrimination of SVA infection in serum samples.

病毒样颗粒（VLP）是具有高度有序的重复结构的高优先级抗原，类似于天然病毒颗粒。我们已经开发了一种竞争性酶联免疫吸附测定（cELISA），用于检测针对Senecavirus A（SVA）的抗体。我们的测定利用在大肠杆菌中表达并组装的SVA VLP表达系统作为包被抗原。VLP比完整的病毒颗粒或肽具有更好的安全性和免疫原性。基于VLPs的cELISA用于检测从不同养猪场采集的342个血清样品，结果表明其特异性和敏感性分别为100％和94％。使用BIOSTONE AsurDx™塞内卡病毒A（SVA）抗体检测试剂盒和间接免疫荧光检测法进行cELISA的一致性率分别为90.0％和94.2％。因此，这种基于VLPs的cELISA可以有效可靠地用于血清样品中SVA感染的检测和鉴别。