AMB Express ( IF 3.7 ) Pub Date : 2021-01-06 , DOI: 10.1186/s13568-020-01169-2 Daniel P. Roberts , Kaitlyn Selmer , Robert Lupitskyy , Clifford Rice , Jeffrey S. Buyer , Jude E. Maul , Dilip K. Lakshman , Jorge DeSouza
Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this extract, prodigiosin and the serratamolide serrawetin W1, were identified based on mass and collision induced dissociation mass fragmentation spectra. An additional four compounds with M+H+ masses (487, 541, 543, and 571) consistent with serratamolides reported in the literature were also detected. Several other compounds with M+H+ masses of 488, 536, 684, 834, 906, and 908 m/z were detected in this ethanol extract inconsistently over multiple liquid chromatography coupled with tandem mass spectrometry (LC/MS–MS) runs. A purified preparation of prodigiosin provided control of damping-off of cucumber caused by P. ultimum when applied as a seed treatment while ethanol extract of cell mass of strain Tn246, a transposon-mutant-derivative of strain N4-5, did not. Strain Tn246 contained a mini-Tn5 Km insertion in a prodigiosin biosynthetic gene and was deficient in production of prodigiosin. All other compounds detected in N4-5 extract were detected in the Tn246 extract. This is the first report demonstrating that prodigiosin can control a plant disease. Other compounds in ethanol extract of strain N4-5 may contribute to disease control.
中文翻译:
用prodigiosin进行种子处理可控制终极腐霉菌对黄瓜的抑制作用
经证明,粘质沙雷氏菌N4-5株细胞质量的乙醇提取物用于黄瓜种子处理时,可以控制卵菌土土壤传播的植物病原体腐霉,其腐解度与种子处理化学农药相同。一些土壤。根据质量和碰撞诱导的解离质量碎片质谱图,鉴定了该提取物中的两种主要化合物,即prodigiosin和serratamolide serrawetin W1。还检测到另外四种具有M + H +质量的化合物(487、541、543和571),与文献中报道的serratamolides一致。M + H +质量为488、536、684、834、906和908 m / z的其他几种化合物在多次液相色谱-串联质谱(LC / MS-MS)联用中,在乙醇提取物中检测到的含量不一致。当将其作为种子处理剂使用时,纯化的原黄菌素制剂可以控制由终极腐霉引起的黄瓜的脱落,而菌株N4-5的转座子突变体菌株Tn246的细胞团的乙醇提取物则不能。Tn246菌株在prodigiosin生物合成基因中包含一个mini-Tn5 Km插入片段,而prodigiosin的产量却不足。在N4-5提取物中检测到的所有其他化合物都在Tn246提取物中检测到。这是第一个证明prodigiosin可以控制植物病害的报告。N4-5株乙醇提取物中的其他化合物可能有助于控制疾病。