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Temperature modulates immune gene expression in mosquitoes during arbovirus infection
Open Biology ( IF 5.8 ) Pub Date : 2021-01-06 , DOI: 10.1098/rsob.200246
B M C Randika Wimalasiri-Yapa 1, 2 , Roberto A Barrero 3 , Liesel Stassen 1 , Louise M Hafner 1 , Elizabeth A McGraw 4 , Alyssa T Pyke 5 , Cassie C Jansen 6 , Andreas Suhrbier 7 , Laith Yakob 8 , Wenbiao Hu 9 , Gregor J Devine 10 , Francesca D Frentiu 1
Affiliation  

The principal vector of dengue, Zika and chikungunya viruses is the mosquito Aedes aegypti, with its ability to transmit pathogens influenced by ambient temperature. We use chikungunya virus (CHIKV) to understand how the mosquito transcriptome responds to arbovirus infection at different ambient temperatures. We exposed CHIKV-infected mosquitoes to 18, 28 and 32°C, and found that higher temperature correlated with higher virus levels, particularly at 3 days post infection, but lower temperature resulted in reduced virus levels. RNAseq analysis indicated significantly altered gene expression levels in CHIKV infection. The highest number of significantly differentially expressed genes was observed at 28°C, with a more muted effect at the other temperatures. At the higher temperature, the expression of many classical immune genes, including Dicer-2, was not substantially altered in response to CHIKV. The upregulation of Toll, IMD and JAK-STAT pathways was only observed at 28°C. Functional annotations suggested that genes in immune response and metabolic pathways related to energy supply and DNA replication were involved in temperature-dependent changes. Time post infection also led to substantially different gene expression profiles, and this varied with temperature. In conclusion, temperature significantly modulates mosquito gene expression in response to infection, potentially leading to impairment of immune defences at higher temperatures.



中文翻译:

虫媒病毒感染期间温度调节蚊子的免疫基因表达

登革热、寨卡病毒和基孔肯雅热病毒的主要传播媒介埃及伊蚊,具有传播受环境温度影响的病原体的能力。我们使用基孔肯雅病毒 (CHIKV) 来了解蚊子转录组如何在不同环境温度下对虫媒病毒感染做出反应。我们将感染 CHIKV 的蚊子暴露于 18、28 和 32°C,发现较高的温度与较高的病毒水平相关,特别是在感染后 3 天,但较低的温度导致病毒水平降低。RNAseq 分析表明 CHIKV 感染中基因表达水平显着改变。在 28°C 下观察到的显着差异表达基因的数量最多,在其他温度下效果更弱。在较高温度下,许多经典免疫基因的表达,包括Dicer-2, 没有因响应 CHIKV 而发生实质性改变。Toll、IMD 和 JAK-STAT 通路的上调仅在 28°C 下观察到。功能注释表明,与能量供应和 DNA 复制相关的免疫反应和代谢途径中的基因参与了温度依赖性变化。感染后的时间也导致了显着不同的基因表达谱,这随温度而变化。总之,温度显着调节蚊子基因表达以响应感染,在较高温度下可能导致免疫防御受损。

更新日期:2021-01-06
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