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Study of the possibility of increasing the intensity of photochemical processes of riboflavin/UV photocrosslinking of scleral collagen by means of tissue immersion clearing
Quantum Electronics ( IF 0.9 ) Pub Date : 2021-01-05 , DOI: 10.1070/qel17483
M.E. Shvachkina 1 , Yu.V. Kistenev 2 , A.B. Pravdin 1 , D.A. Yakovlev 1
Affiliation  

The possibility of increasing the efficiency of light action during scleral collagen riboflavin/UV photocrosslinking by means of immersion clearing of tissue before UV irradiation is studied. The effectiveness of photo-action is assessed by a decrease in the relative content of the photocrosslinking sensitizer, riboflavin, which is determined from the decay times of tissue fluorescence at different stages of the photocrosslinking process. The fluorescence decay times are measured using a multiphoton tomograph in the mode of time-resolved two-photon fluorescence recording. Studies are performed in vitro on porcine sclera samples. An 88 % aqueous solution of glycerol is used as an immersion clearing agent. Optical clearing is shown to make it possible to increase the intensity of photoinduced conversion of riboflavin into non-fluorescent leukoforms during photochemical reactions preceding collagen crosslinking. According to the experimental data obtained for cases of photocrosslinking without optical clearing and with preliminary optical clearing of tissue under the same irradiation conditions, the content of riboflavin that has not undergone photoconversion in tissue, at depths from 40 to 75 μm, after UV irradiation in the first case is approximately 5.6 times (average over 15 regions of two samples) higher than in the second case.



中文翻译:

研究通过组织浸泡清除增加核黄素/UV光交联巩膜胶原的光化学过程强度的可能性

研究了在紫外线照射前通过浸泡清除组织来提高巩膜胶原核黄素/紫外线光交联过程中光作用效率的可能性。光作用的有效性通过光交联敏化剂核黄素的相对含量的降低来评估,这由光交联过程不同阶段组织荧光的衰减时间确定。使用多光子断层扫描仪以时间分辨双光子荧光记录模式测量荧光衰减时间。研究在体外进行在猪巩膜样本上。88% 的甘油水溶液用作浸渍清洁剂。在胶原交联之前的光化学反应中,光学清除显示可以增加光诱导核黄素转化为非荧光白细胞形式的强度。根据在相同辐照条件下未进行光学清除和组织初步光学清除的光交联情况获得的实验数据,在 40 至 75 μm 深度的组织中未经光转换的核黄素的含量,在紫外线照射后第一种情况大约是第二种情况的 5.6 倍(两个样本的 15 个区域的平均值)。

更新日期:2021-01-05
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