To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th. 128 three-week-old weaned rats underwent high-fat diet and exercise (60–70% VO2max, 1 h/day, 5 days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sample time points were set on the PND 21st, 35th, 43rd, and 56th. The testicular testosterone and the mRNA content, and protein content of KISS-1 and GPR54 in testis tissue were detected by ELISA, RT-qPCR, and Western blotting. (1) The protein of KISS-1 and GPR54 increased gradually during the growing period. KISS-1 mRNA peaked at 35D and GPR54 peaked at 43D. (2) High-fat diet affected the expression of the KISS-1/GPR54 system in rat testis and reduced the expression level of KISS-1 protein. (3) 60–70% VO2max exercise decreased the KISS-1/GPR54 expression level. Exercise intervention improved testicular development in rats with a high-fat diet. The expression of KISS-1/GPR54 increased during the growing period. High-fat diet can downregulate the protein and gene expression of KISS-1/GPR54 and change the expression trend. 60–70% VO2max exercise decreased the expression of KISS-1/GPR54, which may be involved in the effects of exercise on high-fat dietary sex hormone disorders.

中文翻译：

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高脂饮食和运动对成年大鼠睾丸KISS-1 / GPR54表达的影响

在PND 21日至56日之间，寻找KISS-1和G蛋白偶联受体54在大鼠睾丸中的表达。对128只三周大的断奶大鼠进行高脂饮食和运动（最大摄氧量60–70％，每天1小时，5天/周），并随机分为C，CE，HC或HE组。采样时间点设置在PND的第21、35、43和56号。ELISA，RT-qPCR，Western blotting检测睾丸组织中睾丸睾丸激素，KISS-1和GPR54的mRNA，蛋白质含量。（1）KISS-1和GPR54的蛋白质在生长期逐渐增加。KISS-1 mRNA在35D达到峰值，GPR54在43D达到峰值。（2）高脂饮食影响大鼠睾丸中KISS-1 / GPR54系统的表达，降低KISS-1蛋白的表达水平。（3）60–70％VO2max运动可降低KISS-1 / GPR54表达水平。运动干预可改善高脂饮食大鼠的睾丸发育。在生长期间，KISS-1 / GPR54的表达增加。高脂饮食可以下调KISS-1 / GPR54的蛋白质和基因表达，并改变其表达趋势。60–70％VO2max运动会降低KISS-1 / GPR54的表达，这可能与运动对高脂饮食性激素紊乱的影响有关。