BACKGROUND:Bet v 1 family identified as one major plant food allergen class, is highly homologous to pathogenesis-related protein 10 (PR-10), but its biological function involved in disease resistance is still unclear. OBJECTIVE:This study aims to investigate whether Bet v 1 potential allergens participate in the resistance of berry crops against fungal pathogen. METHODS:Allergenicity of Bet v 1 proteins in strawberry (Fragaria) was evaluated by bioinformatics methods. Their expression in response to anthracnose and between susceptible and resistance varieties was analyzed. RESULTS:19 Bet v 1 homologous proteins were identified and 15 of them were considered as allergen candidates. RNA-seq analysis indicated most of these Fra a 1s expressed in fruits could be largely induced by the invasion of anthracnose pathogen Colletotrichum. The mRNA level of fruit major allergen Fra a 1.05 in the resistant variety Shenyang (SY) was 20∼50 fold higher compared with those in the susceptible cultivar and two diploid wild species. Immunoblotting using Birch (Betula pendula) Bet v 1 allergen-specific IgG antibody confirmed the large-scale accumulation of potential cross-reactive antigens in SY fruit. CONCLUSIONS:Strawberry Bet v 1 potential allergens exhibit their correspondence with anthracnose resistance that might be instructive to future breeding strategies.

中文翻译：

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Bet v 1潜在过敏原与草莓品种的炭疽病抗性有关

背景：Bet v 1家族被确定为一种主要的植物性食物过敏原，与致病相关蛋白10（PR-10）高度同源，但其与疾病抗性有关的生物学功能仍不清楚。目的：本研究旨在调查Bet v 1潜在过敏原是否参与浆果作物对真菌病原体的抗性。方法：采用生物信息学方法评估草莓（草莓）中Bet v 1蛋白的致敏性。分析了它们对炭疽病的反应以及在易感和抗病品种之间的表达。结果：鉴定出19种Bet v 1同源蛋白，其中15种被认为是过敏原候选物。RNA-seq分析表明，水果中表达的大多数这些Fra a 1s可能主要是由炭疽病原菌炭疽菌的入侵诱导的。抗性品种沉阳（SY）的果实主要过敏原Fra a 1.05的mRNA水平比易感品种和两个二倍体野生种高20〜50倍。使用桦木（Betula pendula）Bet v 1过敏原特异性IgG抗体进行的免疫印迹证实了SY果实中潜在交叉反应抗原的大量积累。结论：Strawberry Bet v 1潜在过敏原表现出与炭疽病抗性的对应关系，这可能对将来的育种策略具有指导意义。使用桦木（Betula pendula）Bet v 1过敏原特异性IgG抗体进行的免疫印迹证实了SY果实中潜在交叉反应抗原的大量积累。结论：Strawberry Bet v 1潜在过敏原表现出与炭疽病抗性的对应关系，这可能对将来的育种策略具有指导意义。使用桦木（Betula pendula）Bet v 1过敏原特异性IgG抗体进行的免疫印迹证实了SY果实中潜在交叉反应抗原的大量积累。结论：Strawberry Bet v 1潜在过敏原表现出与炭疽病抗性的对应关系，这可能对将来的育种策略具有指导意义。