BACKGROUND:The adsorption of salivary pellicle proteins onto the material surface is key for denture plaque formation. OBJECTIVE:We aimed to investigate the adsorption of bovine serum albumin (BSA) and mucin (MCN) onto denture base metal materials using a 27-MHz quartz crystal microbalance (QCM) method. METHODS:A gold (Au), titanium (Ti), and cobalt chromium alloy (Co–Cr) sensors were employed for QCM measurements. Adsorbed amounts of BSA or MCN were calculated by observing the frequency decrease, and the apparent reaction rate, kobs, was obtained by the curve fitting of the frequency shift against the adsorption time. RESULTS:The adsorbed amounts of BSA on Ti were significantly lower than those on Au and Co–Cr. For MCN adsorption, Au showed significantly greater amounts of adsorption than Co–Cr. The kobs of Ti for BSA adsorption was significantly smaller than for the Co–Cr. The kobs of Ti, and Co–Cr for MCN adsorption were significantly smaller than for the Au. A clear correlation was not determined between adsorbed amounts of BSA or MCN onto each sensor and the surface topography or contact angles. CONCLUSIONS:The difference of denture base metals and the difference of salivary proteins influences the adsorption behavior of salivary proteins.

中文翻译：

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27MHz石英晶体微量天平对唾液膜蛋白在义齿基体金属上的吸附行为

背景：唾液膜蛋白在材料表面的吸附是义齿菌斑形成的关键。目的：采用27MHz石英晶体微量天平（QCM）法研究牛血清白蛋白（BSA）和粘蛋白（MCN）在义齿母材金属材料上的吸附作用。方法：采用金 (Au)、钛 (Ti) 和钴铬合金 (Co-Cr) 传感器进行 QCM 测量。BSA 或 MCN 的吸附量通过观察频率降低来计算，表观反应速率 kobs 通过频率偏移与吸附时间的曲线拟合得到。结果：钛对BSA的吸附量明显低于Au和Co-Cr。对于 MCN 吸附，Au 的吸附量明显高于 Co-Cr。吸附 BSA 的 Ti 的 kobs 明显小于 Co-Cr。用于 MCN 吸附的 Ti 和 Co-Cr 的 kobs 明显小于 Au。在每个传感器上的 BSA 或 MCN 吸附量与表面形貌或接触角之间没有明确的相关性。结论：义齿基体金属的差异和唾液蛋白的差异会影响唾液蛋白的吸附行为。