Background. Newts have impressive regenerative capabilities, but it remains unclear about the role of epigenetic regulation in regeneration process. We herein investigated histone modifications in newt tail tissue cells following amputation. Methods and Results. Iberian ribbed male newts (6-8 months old) were suffered to about 1.5 cm length of amputation of their tails for initiating regeneration process, and the residual stump of tail tissues was collected for immunohistochemical analysis 3 days later. Compared to the tissue cells of intact tails, c-kit-positive stem cells and PCNA-positive proliferating cells were significantly higher in tails suffered to amputation (). Amputation also significantly induced the acetylation of H3K9, H3K14, and H3K27 in cells of the tails with amputation (), but did not significantly change the methylation of H3K27 (). Conclusion. These results suggest that epigenetic regulation likely involves in newt tail regeneration following amputation.

中文翻译：

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截肢后蝾螈尾组织细胞中组蛋白 H3 修饰的免疫组织化学分析

背景。蝾螈具有令人印象深刻的再生能力，但表观遗传调控在再生过程中的作用仍不清楚。我们在此研究了截肢后蝾螈尾组织细胞中的组蛋白修饰。方法和结果。伊比利亚肋骨雄性蝾螈(6-8个月大)为了启动再生过程，将它们的尾巴截去约1.5厘米长，3天后收集尾巴组织的残余残端进行免疫组织化学分析。与完整尾巴的组织细胞相比，被截肢的尾巴中c-kit阳性干细胞和PCNA阳性增殖细胞显着增加。）。截肢还显着诱导了截肢后尾部细胞中 H3K9、H3K14 和 H3K27 的乙酰化。)，但没有显着改变 H3K27 的甲基化 (）。 结论。这些结果表明，表观遗传调控可能涉及截肢后蝾螈尾巴的再生。