当前位置: X-MOL 学术Nature › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Control of osteoblast regeneration by a train of Erk activity waves
Nature ( IF 64.8 ) Pub Date : 2021-01-06 , DOI: 10.1038/s41586-020-03085-8
Alessandro De Simone 1, 2 , Maya N Evanitsky 1, 2 , Luke Hayden 1, 2 , Ben D Cox 1, 2, 3 , Julia Wang 1, 2 , Valerie A Tornini 1, 2, 4 , Jianhong Ou 1 , Anna Chao 1, 2 , Kenneth D Poss 1, 2, 5, 6 , Stefano Di Talia 1, 2, 7
Affiliation  

Regeneration is a complex chain of events that restores a tissue to its original size and shape. The tissue-wide coordination of cellular dynamics that is needed for proper morphogenesis is challenged by the large dimensions of regenerating body parts. Feedback mechanisms in biochemical pathways can provide effective communication across great distances1,2,3,4,5, but how they might regulate growth during tissue regeneration is unresolved6,7. Here we report that rhythmic travelling waves of Erk activity control the growth of bone in time and space in regenerating zebrafish scales, millimetre-sized discs of protective body armour. We find that waves of Erk activity travel across the osteoblast population as expanding concentric rings that are broadcast from a central source, inducing ring-like patterns of tissue growth. Using a combination of theoretical and experimental analyses, we show that Erk activity propagates as excitable trigger waves that are able to traverse the entire scale in approximately two days and that the frequency of wave generation controls the rate of scale regeneration. Furthermore, the periodic induction of synchronous, tissue-wide activation of Erk in place of travelling waves impairs tissue growth, which indicates that wave-distributed Erk activation is key to regeneration. Our findings reveal trigger waves as a regulatory strategy to coordinate cell behaviour and instruct tissue form during regeneration.



中文翻译:

通过一系列 Erk 活动波控制成骨细胞再生

再生是一个复杂的事件链,可将组织恢复到其原始大小和形状。适当形态发生所需的细胞动力学的组织范围内的协调受到再生身体部位的大尺寸的挑战。生化途径中的反馈机制可以提供跨远距离的有效交流1,2,3,4,5,但它们如何在组织再生过程中调节生长尚未解决6,7. 在这里,我们报告说,Erk 活动的有节奏的行波在斑马鱼鳞片的再生过程中控制骨骼在时间和空间上的生长,这是毫米大小的保护性防弹衣圆盘。我们发现 Erk 活动波在成骨细胞群中传播,作为从中心源传播的扩展同心环,诱导组织生长的环状模式。结合理论和实验分析,我们表明 Erk 活动作为可激发的触发波传播,能够在大约两天内穿过整个尺度,并且波产生的频率控制着尺度再生的速率。此外,定期诱导同步的、全组织范围内的 Erk 激活代替行波会损害组织生长,这表明波分布的 Erk 激活是再生的关键。我们的研究结果揭示了触发波作为一种调节策略,可在再生过程中协调细胞行为并指导组织形成。

更新日期:2021-01-06
down
wechat
bug