Expression and Clinical Significance of KLRG1 and 2B4 on T Cells in the Peripheral Blood and Tumour of Patients with Cervical Cancer,Immunological Investigations

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Expression and Clinical Significance of KLRG1 and 2B4 on T Cells in the Peripheral Blood and Tumour of Patients with Cervical Cancer
Immunological Investigations ( IF 2.8 ) Pub Date : 2021-01-06 , DOI: 10.1080/08820139.2020.1867567
Yuping Guo _{1,

2,

3} , Yaning Feng _{1,

2,

3} , Peiwen Fan _{1,

2,

3} , Xuan Yao ₄ , Yanchun Peng ₄ , Ruozheng Wang _{1,

2,

3} , Gulina Kuerban _{1,

2,

3}

Affiliation

ABSTRACT

Background

Killer cell lectin-like receptor G1 (KLRG1) and 2B4 play important roles in the immune regulation and immune tolerance to tumor cells by inhibiting T cell function. However, the clinical relevance of KLRG1 and 2B4 to cervical cancer remains to be understood.

Methods

We measured the frequency of KLRG1+ or 2B4+ cells in CD4+ or CD8 + T cells derived from peripheral blood or tumour biopsies in cervical cancer patients by flow cytometry.

Results

Compared with healthy controls, the level of KLRG1 and 2B4 on CD8 + T cells in the blood of the patients increased significantly (P = .0056 and .0441). KLRG1 level on CD8 + T cells and 2B4 level on CD4 + T cells in peripheral blood were significantly higher than in tumor tissues (P < .0001 and P = .0003). Higher KLRG1 level on blood-derived CD8 + T cells was observed in patients older than 54 years (P = .001) or tested to be HPV-negative (P = .026). Tumor-infiltrated CD8 + T cells demonstrated elevated KLRG1 level in patients having pelvic lymph node metastasis (P = .016). Increased 2B4 level on blood-derived CD8 + T cells was also observed in patients older than 54 years (P < .001). KLRG1 expression on both CD4 + T (P = .0158) and CD8 + T (P = .0187) cells in the peripheral blood increased after radiotherapy.

Conclusion

KLRG1 level on T cells was related to age and HPV in patients with cervical cancer, while 2B4 level on T cells was related to age, underlying their roles in the host immune response to cervical cancer. Radiotherapy can improve the immune function of patients.

中文翻译：

KLRG1、2B4对宫颈癌患者外周血及肿瘤T细胞的表达及临床意义

摘要

背景

杀伤细胞凝集素样受体 G1 (KLRG1) 和 2B4 通过抑制 T 细胞功能在肿瘤细胞的免疫调节和免疫耐受中发挥重要作用。然而，KLRG1 和 2B4 与宫颈癌的临床相关性仍有待了解。

方法

我们通过流式细胞术测量了来自宫颈癌患者外周血或肿瘤活检的 CD4+ 或 CD8+ T 细胞中 KLRG1+ 或 2B4+ 细胞的频率。

结果

与健康对照组相比，患者血液中CD8+T细胞上KLRG1和2B4的水平显着升高（P =0.0056和0.0441）。外周血中 CD8 + T 细胞上的 KLRG1 水平和 CD4 + T 细胞上的 2B4 水平显着高于肿瘤组织（P < .0001 和P = .0003）。在 54 岁以上 ( P = .001) 或检测为 HPV 阴性 ( P = .026) 的患者中观察到血液来源的 CD8 + T 细胞上 KLRG1 水平较高。肿瘤浸润的 CD8 + T 细胞在盆腔淋巴结转移患者中表现出 KLRG1 水平升高 ( P = .016)。在 54 岁以上的患者中也观察到血源性 CD8 + T 细胞的 2B4 水平升高（P < .001)。放疗后外周血 CD4 + T ( P = .0158) 和 CD8 + T ( P = .0187) 细胞上的 KLRG1 表达增加。