A method was established for the simultaneous determination of 20 kinds of bile acids in human serum employing ultraperformance liquid chromatography–tandem mass spectrometry. Chromatographic conditions and sample preparation were optimized to achieve good separation and maximum sensitivity for these analytes. The linearity, accuracy and repeatability of the development method were validated with a series of experiments. Under the optimum conditions, good linearities were achieved in the quantitative range for each bile acid with the correlation coefficients (r²) >0.9901. The limit of detections (signal–noise ratio 3) of the method were in a range from 0.02 to 0.57 nmol/L. The recoveries were in the range of 88.1–109.9%, RSD < 6.12%. This method was successfully applied for the determination of bile acids in a human serum sample with simple operation, high sensitivity and good accuracy, and provides a reference for the clinical determination of bile acid content.

中文翻译：

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超高效液相色谱-串联质谱法快速测定人血清中20种胆汁酸

建立了一种同时使用超高效液相色谱-串联质谱法同时测定人血清中20种胆汁酸的方法。对色谱条件和样品制备进行了优化，以实现这些分析物的良好分离和最大灵敏度。通过一系列实验验证了开发方法的线性，准确性和可重复性。在最佳条件下，每种胆汁酸在定量范围内均具有良好的线性关系，相关系数为（r ²）> 0.9901。该方法的检出限（信噪比3）在0.02至0.57 nmol / L的范围内。回收率在88.1–109.9％范围内，RSD <6.12％。该方法操作简便，灵敏度高，准确度高，已成功应用于人血清中胆汁酸的测定，为临床测定胆汁酸含量提供了参考。