Biomaterials Advances ( IF 7.9 ) Pub Date : 2021-01-06 , DOI: 10.1016/j.msec.2020.111840 Sarah Lehnert , Pawel Sikorski
The application of microspheres instead of bulk hydrogels in cell-laden biomaterials offers multiple advantages such as a high surface-to-volume-ratio and, consequently, a better nutrition and oxygen transfer to and from cells. The preparation of inert alginate microspheres is facile, quick, and well-established and the fabrication of alginate-collagen microspheres has been previously reported. However, no detailed characterization of the collagen fibrillogenesis in the alginate matrix is available. We use second-harmonic imaging microscopy reflection confocal microscopy and turbidity assay to study the assembly of collagen in alginate microspheres. We show that the assembly of collagen fibers in a gelled alginate matrix is a complex process that can be aided by addition of small polar molecules, such as glycine and by a careful selection of the gelling buffer used to prepare alginate hydrogels.
中文翻译:
调整藻酸盐微球中胶原纤维的组装
在载有细胞的生物材料中,微球代替散装水凝胶的应用具有多种优势,例如高的表面积与体积之比,因此,可以更好地向细胞和从细胞中转移出更多的营养和氧气。惰性藻酸盐微球的制备是容易,快速和完善的,先前已经报道了藻酸盐-胶原微球的制备。然而,在藻酸盐基质中胶原纤维原纤维形成的详细表征尚不可用。我们使用二次谐波成像显微镜,反射共聚焦显微镜和浊度测定法研究藻酸盐微球中胶原蛋白的组装。我们表明,胶凝藻酸盐基质中胶原纤维的组装是一个复杂的过程,可以通过添加小极性分子来辅助,