The optical polarization properties of virus-infected cells have been measured, analyzed, and compared with the uninfected cells. In this regard, Vero and HeLa cells have been used as the host for Herpes simplex viruses (HSV). By using polarization microscopy imaging technique, the Mueller matrix images of infected and uninfected cells have been recorded. Through image processing and further analysis, the polarization properties of host cells are compared with their infected ones. For quantitative analysis, the multispectral Mueller matrix transformation (MMT) parameters (A and b) are calculated to identify the microstructural differentiations between uninfected and infected cells by HSV. The results of our study show that the structural dependent polarization parameters of A and b are sensitive to the infection situation while, the effect of infection on different cells could be distinguished through these parameters accordingly. The polarimetric microscopy imaging and extracted polarization parameters could be considered as an impressive competitor for time-consuming, expensive, and operator dependent available techniques.

已测量，分析病毒感染的细胞的光偏振特性，并将其与未感染的细胞进行比较。在这方面，Vero和HeLa细胞已用作单纯疱疹病毒（HSV）的宿主。通过使用偏振显微镜成像技术，已感染和未感染细胞的穆勒矩阵图像已被记录。通过图像处理和进一步分析，将宿主细胞的极化特性与其感染的细胞进行比较。为了进行定量分析，计算了多光谱Mueller矩阵变换（MMT）参数（A和b），以通过HSV识别未感染细胞和感染细胞之间的微观结构差异。我们的研究结果表明，A和b的结构相关极化参数对感染情况敏感，而 通过这些参数可以区分感染对不同细胞的影响。偏振显微镜成像和提取的偏振参数可以被认为是费时，昂贵和依赖于操作员的可用技术的令人印象深刻的竞争者。