The fluorescence sensing of specific cationic species is necessary and crucial due to their numerous potential effects on living organisms and the environment. In the present investigation, an efficient dual-channel responsive novel probe based rhodamine 6G condensed isophorone moiety (RHI) was designed and synthesized for sensitive, rapid and specific sensing of metal ions. RHI enabled selective and quick recognition of Zn²⁺ and/or Hg²⁺ in the presence of other tested metal ions in EtOH/H₂O (8/2, v/v). It demonstrated remarkable red for Zn²⁺ or yellow emission enhancement for Hg²⁺ and good photochromism. Addition of S²⁻ to a solution of RHI-M²⁺ reversed the process, followed by quenching emission, enabling reversible use of RHI. It was able to detect the levels of Zn²⁺ and Hg²⁺ within the limit of detection of 82.2 nM and 1.13 μM, respectively. Measurements of mineral and tap water samples prove the potential of the probe RHI for the detection of Zn²⁺ and Hg²⁺ ions in environmental aqueous samples. Dual-channel fluorescence response of RHI for Zn²⁺ and Hg²⁺ ensured a chance to apply them for build simple or complex molecular logic circuits. Moreover, it could easily visualize Zn²⁺ or Hg²⁺ in DDL-1 cells in bioimaging experiments.

由于特定阳离子物种对生物体和环境的众多潜在影响，因此对它们进行荧光传感是必要且至关重要的。在当前的研究中，有效的双通道响应新型探针基于罗丹明6G浓缩的异佛尔酮部分（RHI）的设计和合成，用于金属离子的灵敏，快速和特异性感测。RHI能够在EtOH / H ₂ O（8 / 2，v / v）中存在其他测试金属离子的情况下，选择性和快速识别Zn ²⁺和/或Hg ²⁺。Zn ²⁺具有明显的红色，Hg ²⁺具有增强的黄色发光，并具有良好的光致变色性。将S ²⁻加到RHI-M的解中²⁺逆转了这一过程，随后淬灭了发射，从而可逆地使用RHI。它能够在分别为82.2 nM和1.13μM的检出限内检测到Zn ²⁺和Hg ²⁺的水平。矿物质和自来水样品的测量证明了探针RHI在检测环境水性样品中Zn ²⁺和Hg ²⁺离子方面的潜力。RHI对Zn ²⁺和Hg ^2+的双通道荧光响应确保了将其应用于构建简单或复杂的分子逻辑电路的机会。此外，在生物成像实验中，它可以轻松地可视化DDL-1细胞中的Zn ²⁺或Hg ²⁺。