Nature has gifted us many organic molecules which have remarkable influence in our daily life. Amongst many organic molecules, heterocyclic organic molecules have gained potential applications in the advanced field of biomedicine, pharmaceutical, electronics and many more. In the present work fluorescence quenching of biologically active fluorescent probe 8EMOHCC by aniline in different solvents have been studied at room temperature. To understand the molecular behaviour in different media, solvents of different refractive index and dielectric constant have been used. Spectroscopic measurement techniques such as UV/Vis spectroscopy and time related single photon counting are employed to characterise the molecule at room temperature. The fluorescence quenching study shows linear dependence of SV-plot in solvents of different dielectric constants. It reveals that quenching reactions are dynamic in nature. Various parameters of quenching have been determined and identified the type of quenching involved in the quenching reaction. Further, k_q is found to be greater than \( {k}_q^{\prime } \) in ACN, methanol, propanol and dioxane. Activation energy of quenching (E_a) is found to be greater than energy of diffusion (E_d) in ACN, methanol, propanol, THF solvents and E_d > E_a in dioxane, indicating that quenching reaction is not solely controlled by material diffusion but also activation process.

大自然赐予我们许多有机分子，它们对我们的日常生活有着显着的影响。在众多有机分子中，杂环有机分子在生物医学、制药、电子等先进领域获得了潜在的应用。在目前的工作中，研究了室温下苯胺在不同溶剂中对生物活性荧光探针 8EMOHCC 的荧光猝灭。为了了解不同介质中的分子行为，使用了不同折射率和介电常数的溶剂。光谱测量技术如紫外/可见光谱和时间相关的单光子计数被用来在室温下表征分子。荧光猝灭研究显示了在不同介电常数的溶剂中 SV 曲线的线性相关性。它揭示了淬灭反应本质上是动态的。已经确定了淬灭的各种参数并确定了淬灭反应中涉及的淬灭类型。更远，在乙腈、甲醇、丙醇和二恶烷中发现k _q大于\( {k}_q^{\prime } \)。发现在乙腈、甲醇、丙醇、四氢呋喃溶剂中猝灭活化能 ( E _a ) 大于扩散能 ( E _d )，在二恶烷中E _d  >  E _a表明猝灭反应不仅仅受材料扩散的控制还有激活过程。