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A systematic review of the Uridine diphosphate-Galactose/Glucose-4-epimerase (UGE) in plants
Plant Growth Regulation ( IF 4.2 ) Pub Date : 2021-01-06 , DOI: 10.1007/s10725-020-00686-1
Jiaming Hou , Shaokai Tian , Lin Yang , Zhixin Zhang , Ying Liu

Uridine diphosphate (UDP)-Galactose/Glucose-4-epimerase (UGE) is the third enzyme involved in the Leloir pathway. It catalyzes the conversion of UDP-Galactose to UDP-Glucose, which is a rate-limiting step for polysaccharides biosynthesis. As the main cell wall materials, polysaccharides play an irreplaceable role throughout the whole life history of plant. In this review, 1243 UGE mRNA sequences registered in NCBI were obtained and their evolutionary relationship was analyzed by constructing a neighbor-joining tree based on representative sequences. The physicochemical properties of 15 specific UGEs were analyzed, and four UGE sequences of Setaria italica, Zea mays, Ricinus Communis, and Oryza sativa were selected for further secondary structure, three-dimensional protein modeling, transmembrane structure, and signal peptide prediction analyses. The results demonstrate that UGEs are mostly conservative without signal peptides and exert activities in cytoplasm. Then, studies related to the catalytic reaction, characteristic structure, function, and regulation of UGE were summarized, which provide strong evidence that UGE plays a crucial role in cell growth, cell differentiation, cell-to-cell communication, primary metabolism, secondary metabolism, and defense responses. However, the molecular mechanisms whereby UGE regulates the plant stress resistance and useful secondary metabolites accumulation are far from clear. This paper will lay a foundation for further studies and applications of UGE.



中文翻译:

对植物中尿苷二磷酸半乳糖/葡萄糖-4-表异构酶(UGE)的系统评价

尿苷二磷酸(UDP)-半乳糖/葡萄糖-4-表观异构酶(UGE)是参与Leloir途径的第三个酶。它催化UDP-半乳糖向UDP-葡萄糖的转化,这是多糖生物合成的限速步骤。作为主要的细胞壁材料,多糖在植物的整个生命过程中都扮演着不可替代的角色。本文综述了在NCBI中注册的1243个UGE mRNA序列,并通过基于代表序列构建邻居连接树来分析了它们的进化关系。分析了15个特定UGE的理化性质,并分析了Setaria italicaZea maysRicinus CommunisOryza sativa的4个UGE序列。选择用于进一步的二级结构,三维蛋白质建模,跨膜结构和信号肽预测分析。结果表明,UGEs大多数是保守的,没有信号肽,并在细胞质中发挥作用。然后,总结了与UGE的催化反应,特征结构,功能和调控有关的研究,这些有力的证据表明UGE在细胞生长,细胞分化,细胞间通讯,一级代谢,二级代谢中起着至关重要的作用。 ,以及防御反应。但是,UGE调节植物抗逆性和有用的次生代谢产物积累的分子机制尚不清楚。本文将为UGE的进一步研究和应用奠定基础。

更新日期:2021-01-06
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