Objective

Esophageal squamous cell carcinoma (ESCC) is featured by early metastasis and late diagnosis. MicroRNA-301 (miR-301) is known to participate in diverse cancers. Nevertheless, effects of miR-301 on ESCC remain unexplored. Thus, we aim to explore the role of miR-301 in ESCC progression.

Methods

Expression of miR-301 and phosphatase and tensin homologue (PTEN) in ESCC tissues and cell lines was assessed. Next, the screened cells were treated with altered miR-301 or PTEN oligonucleotide and plasmid, and then, the colony formation ability, cell viability, migration, invasion, cell cycle distribution and apoptosis of ESCC cells were assessed. Moreover, tumor growth and microvessel density (MVD) were also assessed, and the targeting relationship between miR-301 and PTEN was affirmed.

Results

MiR-301 was upregulated, and PTEN was downregulated in ESCC tissues and cells. KYSE30 cells and Eca109 cells were selected for functional assays. In KYSE30 cells, inhibited miR-301 or overexpressed PTEN suppressed cell malignant behaviors, and silenced PTEN eliminated the impact of miR-301 inhibition on ESCC progression. In Eca109 cells, miR-301 overexpression or PTEN inhibition promoted cell malignant behaviors, and PTEN overexpression reversed the effects of miR-301 elevation on ESCC progression. The in vivo assay revealed that miR-301 inhibition or PTEN overexpression repressed ESCC tumor growth and MVD, and miR-301 elevation or PTEN reduction had contrary effects. Moreover, PTEN was targeted by miR-301.

Conclusion

Taken together, results in our study revealed that miR-301 affected cell growth, metastasis and angiogenesis via regulating PTEN expression in ESCC.

中文翻译：

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受抑制的 MicroRNA-301 通过升高 PTEN 抑制食管鳞状细胞癌的血管生成和细胞生长

客观的

食管鳞状细胞癌（ESCC）具有转移早、诊断晚的特点。已知 MicroRNA-301 (miR-301) 参与多种癌症。然而，miR-301 对 ESCC 的影响仍有待探索。因此，我们的目的是探讨 miR-301 在 ESCC 进展中的作用。

方法

评估了 ESCC 组织和细胞系中 miR-301 以及磷酸酶和张力蛋白同源物 (PTEN) 的表达。接下来，用改变的miR-301或PTEN寡核苷酸和质粒处理筛选的细胞，然后评估ESCC细胞的集落形成能力、细胞活力、迁移、侵袭、细胞周期分布和凋亡。此外，还评估了肿瘤生长和微血管密度（MVD），并肯定了miR-301和PTEN之间的靶向关系。

结果

在 ESCC 组织和细胞中，MiR-301 上调，PTEN 下调。选择KYSE30细胞和Eca109细胞进行功能测定。在 KYSE30 细胞中，抑制 miR-301 或过表达 PTEN 可抑制细胞恶性行为，而沉默 PTEN 则消除了 miR-301 抑制对 ESCC 进展的影响。在Eca109细胞中，miR-301过表达或PTEN抑制促进细胞恶性行为，而PTEN过表达逆转了miR-301升高对ESCC进展的影响。体内测定显示，miR-301抑制或PTEN过表达可抑制ESCC肿瘤生长和MVD，而miR-301升高或PTEN减少则具有相反的作用。此外，PTEN 是 miR-301 的靶标。

结论

综上所述，我们的研究结果表明，miR-301 通过调节 ESCC 中 PTEN 的表达来影响细胞生长、转移和血管生成。