Conductive and degradable nanofibrous scaffolds have great potential in promoting cell growth, proliferation, and differentiation under an external electric field. Although the issue of inferior electrical conductivity in body fluids still exists, polyaniline (PANI)-based degradable nanofibers can promote cell adhesion, growth, and proliferation. To investigate whether the effect is caused by the PANI morphology, we selected three inorganic acids as dopants in the process of PANI in situ oxidative polymerization: hydrochloric acid, sulfuric acid, and perchloric acid. The obtained polyaniline/polylactic acid (PANI/PLA) composite nanofibers were characterized via SEM, FTIR, and XPS analysis, and we confirmed that the PLA nanofibers were successfully coated by PANI without any change to the porous structure of the PLA nanofibers. The in vitro mechanical properties and degradability indicated that the oxidation of acid dopants should be considered and that it was likely to have a higher oxidation degradation effect on PLA nanofibers. The contact angle test demonstrated that PANI/PLA composite nanofibers with different surface morphologies have good wettability, implying that they meet the requirements of bone tissue engineering scaffolds. The surface roughness and cell viability demonstrated that different PANI morphologies on the surface can promote cell proliferation. The higher the surface roughness of the PANI, the better the biocompatibility. Consequently, the regulated surface morphology of PANI/PLA composite nanofibers via different acids doping has positive effect on biocompatibility in tissue engineering.

导电和可降解的纳米纤维支架在外电场下具有促进细胞生长，增殖和分化的巨大潜力。尽管体液中导电性较差的问题仍然存在，但基于聚苯胺（PANI）的可降解纳米纤维可以促进细胞粘附，生长和增殖。为了研究这种影响是否是由PANI形态引起的，我们在PANI原位氧化聚合过程中选择了三种无机酸作为掺杂剂：盐酸，硫酸和高氯酸。通过SEM，FTIR和XPS分析对获得的聚苯胺/聚乳酸（PANI / PLA）复合纳米纤维进行了表征，我们证实PANI纳米纤维已成功被PANI包覆，而PLA纳米纤维的多孔结构没有任何变化。体外机械性能和可降解性表明应考虑酸掺杂剂的氧化，并且它可能对PLA纳米纤维具有更高的氧化降解作用。接触角测试表明，具有不同表面形态的PANI / PLA复合纳米纤维具有良好的润湿性，表明它们满足骨组织工程支架的要求。表面粗糙度和细胞活力证明，表面上不同的PANI形态可以促进细胞增殖。PANI的表面粗糙度越高，生物相容性越好。因此，通过不同的酸掺杂来调节PANI / PLA复合纳米纤维的表面形态对组织工程中的生物相容性具有积极作用。