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Lactococcus lactis subsp. cremoris of Plant Origin Produces Antifungal Cyclo-(Leu-Pro) and Tetradecanoic Acid
Indian Journal of Microbiology ( IF 3 ) Pub Date : 2021-01-06 , DOI: 10.1007/s12088-020-00917-z
Milind Gajbhiye 1 , Balu Kapadnis 2
Affiliation  

The antifungal cyclo-depeptide and the fatty acid were isolated and purified from an indigenous strain of Lactococcus lactis subsp. cremoris. Maximal activity was observed at pH 5.5 and 6.5, and at 30 °C under stationary conditions, which was detected in the culture supernatant 8 h post-inoculation in MRS broth until 22 h. The activity of antifungal compounds in the culture supernatant was sensitive to pH and temperature; and was protease-resistant. The antifungal compounds were concentrated by freeze-drying and ultrafiltration with activity retained in 1 kDa filtrates indicating low molecular weight metabolites. The compounds were further extracted by using different solvents amongst which, ethyl acetate provided the highest recovery. Antifungal compounds were separated on a silica gel column into two active fractions that were revealed to be tetradecanoic acid and cyclo-(Leu-Pro), a cyclic dipeptide, by GC–MS. Herein, we describe and attribute the biocontrol potential of L. lactis subsp. cremoris to the low molecular weight antifungal compounds isolated, which is the first report of their isolation from this strain. The broad antifungal spectrum of this candidate advocates further exploration of its biocontrol potential in managing fungal infections in different food and feed systems.



中文翻译:

乳酸乳球菌亚种。植物来源的 cremoris 产生抗真菌环-(Leu-Pro) 和十四烷酸

从乳酸乳球菌亚种的本地菌株中分离和纯化抗真菌环肽和脂肪酸。克雷莫里斯。在 pH 5.5 和 6.5 以及 30 °C 下在静止条件下观察到最大活性,在 MRS 肉汤中接种后 8 小时至 22 小时,在培养上清液中检测到该活性。培养上清液中抗真菌化合物的活性对pH和温度敏感;并且是蛋白酶抗性的。通过冷冻干燥和超滤浓缩抗真菌化合物,其活性保留在 1 kDa 滤液中,表明低分子量代谢物。通过使用不同的溶剂对化合物进行进一步萃取,其中乙酸乙酯的回收率最高。抗真菌化合物在硅胶柱上分离成两个活性部分,GC-MS 显示它们是十四烷酸和环-(Leu-Pro),一种环状二肽。在这里,我们描述和归因于生物防治潜力L. lactis subsp. cremoris中分离出低分子量的抗真菌化合物,这是首次报道它们从该菌株中分离出来。该候选药物的广泛抗真菌谱提倡进一步探索其在管理不同食品和饲料系统中的真菌感染方面的生物防治潜力。

更新日期:2021-01-06
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