当前位置: X-MOL 学术Funct. Integr. Genomics › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Long-distance chromatin interaction of IGF1 during embryonic and postnatal development in the liver of Sus scrofa
Functional & Integrative Genomics ( IF 2.9 ) Pub Date : 2021-01-06 , DOI: 10.1007/s10142-020-00761-w
Jinbi Zhang 1, 2 , Wang Yao 2 , Zengxiang Pan 2 , Honglin Liu 2
Affiliation  

The dynamics of chromatin have been the focus of studies aimed at characterizing gene regulation. Among various chromosome conformation capture methods, 4C-seq is a powerful technique to identify genome-wide interactions with a single locus of interest. Insulin-like growth factor 1 (IGF1) is a member of the somatotropin axis that plays a significant role in cell proliferation and growth. Determining the IGF1-involved genome-wide chromatin interaction profile at different growth stages not only is important for understanding IGF1 transcriptional regulation but also provides a representation of genome-wide chromatin transformation during development. Using the IGF1 promoter as a “bait”, we identified genome-wide interactomes of embryonic (E70) and postnatal (P1 and P70) pig liver cells by 4C-seq. The IGF1 promoter interactomes varied significantly among the three developmental stages. The most active chromatin interaction was observed in the P1 stage, while the highest interaction variability was observed in the P70 stage. The identified 4C sites were enriched around transcription start sites, CpG sites and functional pig QTLs. In addition, the genes located in the interacting regions and the involved pathways were also analysed. Overall, our work reveals a distinct long-distance regulatory pattern in pig liver during development for the first time, and the identified interacting sites and genes may serve as candidate targets in further transcriptional mechanism studies and effective molecular markers for functional traits.



中文翻译:

野猪肝脏胚胎和出生后发育过程中IGF1的长距离染色质相互作用

染色质的动力学一直是旨在表征基因调控的研究的重点。在各种染色体构象捕获方法中,4C-seq 是一种强大的技术,可以识别与单个感兴趣基因座的全基因组相互作用。胰岛素样生长因子 1 (IGF1) 是生长激素轴的成员,在细胞增殖和生长中起重要作用。确定不同生长阶段IGF1参与的全基因组染色质相互作用谱不仅对于理解IGF1转录调控很重要,而且还提供了发育过程中全基因组染色质转化的代表。使用IGF1启动子作为“诱饵”,我们通过 4C-seq 鉴定了胚胎(E70)和出生后(P1 和 P70)猪肝细胞的全基因组相互作用组。IGF1 启动子相互作用组在三个发育阶段之间存在显着差异。在 P1 阶段观察到最活跃的染色质相互作用,而在 P70 阶段观察到最高的相互作用变异性。鉴定的 4C 位点在转录起始位点、CpG 位点和功能性猪 QTL 周围富集。此外,还分析了位于相互作用区域的基因和涉及的途径。总体而言,我们的工作首次揭示了猪肝在发育过程中的明显长距离调控模式,

更新日期:2021-01-06
down
wechat
bug