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Some recent data on sperm morphology and motility kinetics in Atlantic cod ( Gadus morhua L.)
Fish Physiology and Biochemistry ( IF 2.9 ) Pub Date : 2021-01-06 , DOI: 10.1007/s10695-020-00915-4
Sayyed Mohammad Hadi Alavi 1 , Azadeh Hatef 2 , Ian A E Butts 3 , Olga Bondarenko 4 , Jacky Cosson 4 , Igor Babiak 5
Affiliation  

Studying biology of sperm provides valuable information to optimize artificial reproduction and is crucial for sustainable aquaculture. Here, we investigated morphology of spermatozoon in Atlantic cod (Gadus morhua) using transmission and scanning electron microscopy. Furthermore, spermatozoa motility kinetics at different osmolalities were studied using computer-assisted sperm analysis software. The spermatozoon lacked an acrosome and consisted of a head, midpiece, and flagellum. The head of spermatozoa was round, oval, and rather elongated in shape, showing high variations in dimensions. There were up to 6 mitochondria that encircled the proximal part of the flagellum. The proximal and distal centrioles were located within the nuclear notch and arranged orthogonal to each other. The axoneme had a typical 9 + 2 microtubule structure. The flagellar length of spermatozoon was 66.94 ± 0.46 μm. Spermatozoa were immotile in the seminal plasma. Dilution of sperm with natural seawater (1100 mOsmol/kg) resulted in initiation of motility for 91.0 ± 3.4% of spermatozoa with average velocity of 86.2 ± 2.3 μm/s and beating frequency of 52 Hz. The duration of spermatozoa motility was > 6 min; however, the percentage of motile spermatozoa decreased at 60 s post-activation. When osmolality of natural seawater was modified using distilled water or NaCl, spermatozoa motility was not initiated at ≤ 400 and ≥ 2500 mOsmol/kg, and the highest percentage of motility was observed at 730–1580 mOsmol/kg. In a sucrose solution, spermatozoa motility was initiated and suppressed at 600 and 1500 mOsmol/kg, respectively, and highest percentage of motility was observed at 800–1100 mOsmol/kg. Spermatozoon morphology comparisons within Gadiformes showed differences in dimensions of head and mitochondria, flagellar length, and number of mitochondria. The present study provides valuable data that can be used for phylogenetic implications based on spermatozoon morphology and for development of artificial fertilization and sperm cryopreservation protocols based on sperm motility.



中文翻译:

关于大西洋鳕鱼(Gadus morhua L.)精子形态和运动动力学的一些最新数据

对精子生物学的研究为优化人工繁殖提供了有价值的信息,对于可持续水产养殖至关重要。在这里,我们研究了大西洋鳕鱼(Gadus morhua)中精子的形态)使用透射和扫描电子显微镜。此外,使用计算机辅助的精子分析软件研究了在不同摩尔渗透压浓度下的精子运动动力学。精子缺乏顶体,由头部,中段和鞭毛组成。精子的头部是圆形的,椭圆形的,并且形状相当细长,显示出较大的尺寸差异。围绕鞭毛近端的线粒体多达6个。近端和远端中心体位于核槽口内,并且彼此正交。轴突具有典型的9 + 2微管结构。精子的鞭毛长度为66.94±0.46μm。精子在精浆中不活动。用天然海水(1100 mOsmol / kg)稀释精子会导致活力开始,为91.0±3。4%的精子,平均速度为86.2±2.3μm/ s,跳动频率为52 Hz。精子运动的持续时间> 6分钟;然而,活动性精子的百分比在激活后60 s下降。当使用蒸馏水或NaCl调节天然海水的重量克分子渗透浓度时,在≤400和≥2500 mOsmol / kg时不会启动精子运动,并且在730–1580 mOsmol / kg时观察到最高的运动百分比。在蔗糖溶液中,分别在600和1500 mOsmol / kg下开始并抑制了精子运动,在800-1100 mOsmol / kg下观察到了最高的运动百分比。Gadiformes内的精子形态比较显示,头和线粒体的尺寸,鞭毛长度和线粒体的数量存在差异。

更新日期:2021-01-06
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